Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,562	C→T	D321N (GAT→AAT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,562	0	C	T	100.0%	95.9 / NA	28	D321N (GAT→AAT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

GGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAC  >  NC_000913/4629431‑4629693                                                                                                                                    |                                                                                                                                    ggTCGATTTACCCGCACCGTTCGGACCGATGA‑CCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcg                                                                                                                            <  1:357974/140‑1 (MQ=255)  gTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcg                                                                                                                            <  1:100630/140‑1 (MQ=255)  gTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcg                                                                                                                            >  2:100630/1‑140 (MQ=255)  gTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcg                                                                                                                            <  1:328004/140‑1 (MQ=255)        ttACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAc                                                                                                                             >  1:280229/1‑133 (MQ=255)        ttACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAc                                                                                                                             <  2:280229/133‑1 (MQ=255)          aCCCCCCCCGGTCCGACCGAAGATCCCGACGAACCCTCCTTTCGGGATCGAGAAGCTCAAGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAg                                                                                                                    <  2:4205/140‑1 (MQ=255)                     tCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcgc                                                                                                                           >  1:224146/1‑122 (MQ=255)                     tCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGAcgc                                                                                                                           <  2:224146/122‑1 (MQ=255)                              tgatCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTg                                                                                                <  1:160330/140‑1 (MQ=255)                               gatCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTgg                                                                                               <  2:32686/140‑1 (MQ=255)                                           cGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGtt                                                                                   >  1:359831/1‑140 (MQ=255)                                            gCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGt                                                                                         <  2:362501/133‑1 (MQ=255)                                            gCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGt                                                                                         >  1:362501/1‑133 (MQ=255)                                                 tttCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGat                                                                             >  2:293893/1‑140 (MQ=255)                                                    cGGGATCGAGAAGCTCAGGTCATCAACCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATAtt                                                                          <  1:293893/140‑1 (MQ=255)                                                                      gTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGttctt                                                        >  1:287786/1‑140 (MQ=255)                                                                      gTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGttctt                                                        <  2:287786/140‑1 (MQ=255)                                                                        catcaATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCa                                                      >  1:194814/1‑140 (MQ=255)                                                                           caatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGCTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAg                                                   <  1:282392/140‑1 (MQ=255)                                                                              tcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcgc                                                <  2:359831/140‑1 (MQ=255)                                                                                agcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcgcgc                                              <  2:194814/140‑1 (MQ=255)                                                                                                       aCGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACt                       >  2:308870/1‑140 (MQ=255)                                                                                                       aCGCAGGTTGCTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACt                       >  2:402841/1‑140 (MQ=255)                                                                                                                 cTGACTTCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCttt             <  1:402841/140‑1 (MQ=255)                                                                                                                       tCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAcc       >  2:32986/1‑140 (MQ=255)                                                                                                                       tCCAGCACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAcc       >  1:349974/1‑140 (MQ=255)                                                                                                                            cACTTTATTGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAc  <  1:32986/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    GGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAC  >  NC_000913/4629431‑4629693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑