Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,547	A→G	V32A (GTC→GCC)	yajL ←	protein/nucleic acid deglycase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,547	0	A	G	100.0%	70.4 / NA	20	V32A (GTC→GCC)	yajL	protein/nucleic acid deglycase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

TAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGT  >  NC_000913/443413‑443683                                                                                                                                       |                                                                                                                                         tAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTg                                                                                                                                     >  2:33505/1‑140 (MQ=255)          caGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATAccgccg                                                                                                                            >  2:108937/1‑140 (MQ=255)          caGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATAccgccg                                                                                                                            >  2:16899/1‑140 (MQ=255)                       cACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGAt                                                                                                               <  1:33505/140‑1 (MQ=255)                            cATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCcgcg                                                                                                                          <  1:271230/124‑1 (MQ=255)                            cATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCcgcg                                                                                                                          >  2:271230/1‑124 (MQ=255)                                ttCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAgtgg                                                                                                      >  1:156530/1‑140 (MQ=255)                                        cAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCt                                                                                              <  1:108937/140‑1 (MQ=255)                                              cTTCGACCAGCGGCGCATCCACCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGtc                                                                                        >  1:156748/1‑140 (MQ=255)                                                              aTCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAgggg                                                                        <  1:16899/140‑1 (MQ=255)                                                                                   gcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                   <  2:156748/140‑1 (MQ=255)                                                                                    cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATgc                                                  >  1:330308/1‑140 (MQ=255)                                                                                       gAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                               <  2:330308/140‑1 (MQ=255)                                                                                                      aGGTTACCATCGCTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct                                <  2:156530/140‑1 (MQ=255)                                                                                                                  cTGGCGCCGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCa                    <  1:79542/140‑1 (MQ=255)                                                                                                                  cTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCa                    >  2:265753/1‑140 (MQ=255)                                                                                                                  cTGGCGACGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCa                    >  2:79542/1‑140 (MQ=255)                                                                                                                         cGCTGGCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCaaa             >  2:224383/1‑140 (MQ=255)                                                                                                                              gCAGTGGTGGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc        <  1:224383/140‑1 (MQ=255)                                                                                                                                    gtgGCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtgt  >  2:216668/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGT  >  NC_000913/443413‑443683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑