Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	490,996	T→C	F39L (TTT→CTT)	ybaN →	conserved inner membrane protein YbaN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	490,996	0	T	C	100.0%	55.5 / NA	16	F39L (TTT→CTT)	ybaN	conserved inner membrane protein YbaN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/7);  total (9/7)

TCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCG  >  NC_000913/490859‑491095                                                                                                                                          |                                                                                                    tctGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCctt                                                                                                   >  1:205591/1‑140 (MQ=255)           gACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGttctt                                                                                         >  2:273136/1‑140 (MQ=255)                              cGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCcgc                                                                                   >  2:325285/1‑127 (MQ=255)                              cGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCcgc                                                                                   <  1:325285/127‑1 (MQ=255)                                      tttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGt                                                              >  2:121713/1‑140 (MQ=255)                                       ttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTa                                                             <  1:267583/140‑1 (MQ=255)                                       ttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTa                                                             <  1:273136/140‑1 (MQ=255)                                                         ctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAg                                           <  1:121713/140‑1 (MQ=255)                                                         ctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAg                                           >  2:317316/1‑140 (MQ=255)                                                               gTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCt                                     >  2:102784/1‑140 (MQ=255)                                                                                       gtattaccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGc             >  2:418776/1‑140 (MQ=255)                                                                                             ccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGaa                      >  2:21215/1‑125 (MQ=255)                                                                                             ccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGaa                      >  2:269147/1‑125 (MQ=255)                                                                                             ccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGaa                      <  1:269147/125‑1 (MQ=255)                                                                                             ccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGaa                      <  1:21215/125‑1 (MQ=255)                                                                                                 tattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCcgcgc   >  1:393285/1‑140 (MQ=255)                                                                                                  attaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCCTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCcgcgcg  <  1:102784/140‑1 (MQ=255)                                                                                                                                          |                                                                                                    TCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCG  >  NC_000913/490859‑491095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑