Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,958	T→C	S186P (TCC→CCC)	fixA →	putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,958	0	T	C	100.0%	35.8 / NA	11	S186P (TCC→CCC)	fixA	putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

ACGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACTCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCAGAACA  >  NC_000913/42833‑43067                                                                                                                              |                                                                                                              aCGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCt                                                                                                 <  1:174204/140‑1 (MQ=255)                         tGACGGCAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTcggcgcg                                                                         >  2:147400/1‑140 (MQ=255)                             ggCAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGa                                                                     >  1:188436/1‑140 (MQ=255)                               cAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGaaa                                                                   >  2:303306/1‑140 (MQ=255)                                     cccTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCGACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGccc                                                             >  1:268629/1‑140 (MQ=255)                                       cTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGt                                                           <  1:172792/140‑1 (MQ=255)                                       cTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGt                                                           <  2:268629/140‑1 (MQ=255)                                        tCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTc                                                          <  1:170558/140‑1 (MQ=255)                                                                          aaccTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACg                        >  2:344762/1‑140 (MQ=255)                                                                                                gcGGTTGTTGCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCAGaaca  <  1:147400/140‑1 (MQ=255)                                                                                                cctgcggttgCTGTTTCCACTGATATCAACCCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCAGaaca  >  1:223780/7‑140 (MQ=255)                                                                                                                              |                                                                                                              ACGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAGCGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACTCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCAGAACA  >  NC_000913/42833‑43067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑