Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	779,742	T→C	S49P (TCT→CCT)	cpoB →	cell division coordinator CpoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	779,742	0	T	C	100.0%	48.0 / NA	14	S49P (TCT→CCT)	cpoB	cell division coordinator CpoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

TCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACTGAATCAGGTCGTGGAGCGGCAAAAGC  >  NC_000913/779630‑779874                                                                                                                 |                                                                                                                                     tCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAAccc                                                                                                           >  1:408512/1‑140 (MQ=255)                   tataGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACttt                                                                                         >  1:125419/1‑140 (MQ=255)                                 gggCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc                                                                           <  1:271639/140‑1 (MQ=255)                                             cTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTcc                                                               <  2:408512/140‑1 (MQ=255)                                                           agtagtGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGAt                                                 <  2:125419/140‑1 (MQ=255)                                                                  tCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGaa                                          >  2:162029/1‑140 (MQ=255)                                                                    ggCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGaaaa                                        <  1:36118/140‑1 (MQ=255)                                                                     gCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAAt                                       >  2:337868/1‑140 (MQ=255)                                                                               ggTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAAc                             >  2:232056/1‑140 (MQ=255)                                                                                gTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACt                            >  1:120689/1‑140 (MQ=255)                                                                                gTCGAAGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACt                            >  1:203778/1‑140 (MQ=255)                                                                                     aGACCGCGTCACTCAACTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACTGAATc                       >  2:89513/1‑140 (MQ=255)                                                                                                     cTTGAGCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACTGAATCAGGTCGTGGAGCGGCa       <  1:89513/140‑1 (MQ=255)                                                                                                          gCGTATTCCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACTGAATCAGGTCGTGGAGCGGCAAAAGc  <  2:203778/140‑1 (MQ=255)                                                                                                                 |                                                                                                                                     TCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGGAAAATCAGTATCAACTGAATCAGGTCGTGGAGCGGCAAAAGC  >  NC_000913/779630‑779874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑