Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,034,314	A→G	Y354C (TAT→TGT)	hyaB →	hydrogenase 1 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,034,314	0	A	G	100.0%	90.2 / NA	26	Y354C (TAT→TGT)	hyaB	hydrogenase 1 large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

TGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTATCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCG  >  NC_000913/1034183‑1034441                                                                                                                                    |                                                                                                                                tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  2:53205/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  2:312410/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  1:22200/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  1:328676/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  1:371895/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  1:91701/1‑140 (MQ=255) tGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGCGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATc                                                                                                                         >  1:319668/1‑140 (MQ=255)         aCTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGAt                                                                                                                 <  1:22342/140‑1 (MQ=255)              ggCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCTGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGTCTGGTGTAGATATCCCAACGATCAGGt                                                                                                            <  2:211320/140‑1 (MQ=255)                                   ccTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATgg                                                                                       >  1:281092/1‑140 (MQ=255)                                   ccTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATgg                                                                                       <  2:245536/140‑1 (MQ=255)                                     tGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCa                                                                                     <  2:281092/140‑1 (MQ=255)                                       gcggcgCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATgg                                                                                       >  1:208197/1‑136 (MQ=255)                                       gcggcgCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATgg                                                                                       <  2:208197/136‑1 (MQ=255)                                                   ttAACGGCGACTTCAACAATGCGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTgg                                                                       <  2:319668/140‑1 (MQ=255)                                                     aaCGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTa                                                                     <  1:312410/140‑1 (MQ=255)                                                                caacaaTGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCg                                                          <  2:111721/140‑1 (MQ=255)                                                                          ctgcCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCg                                                          >  2:248185/1‑130 (MQ=255)                                                                          ctgcCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCg                                                          <  1:248185/130‑1 (MQ=255)                                                                          ctgcCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAgg                                                >  1:210741/1‑140 (MQ=255)                                                                                gTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGa                                          >  2:192533/1‑140 (MQ=255)                                                                                   gATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATAc                                       <  1:73519/140‑1 (MQ=255)                                                                                   gATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATAc                                       <  2:91701/140‑1 (MQ=255)                                                                                        ggTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACa                                  <  2:22200/140‑1 (MQ=255)                                                                                                    gcagGTGCAGGAGTTTGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCtga                      <  1:40434/140‑1 (MQ=255)                                                                                                                  ttGTCGACCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGc        <  1:192533/140‑1 (MQ=255)                                                                                                                        aCCACGCCTGGTGTCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCg  >  2:200506/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                TGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTATCGATATCCCAACGATCAGGTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCG  >  NC_000913/1034183‑1034441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑