Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,101,354	T→C	I111V (ATT→GTT)	csgG ←	curli secretion channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,101,354	0	T	C	100.0%	74.0 / NA	21	I111V (ATT→GTT)	csgG	curli secretion channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

ACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAATAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGG  >  NC_000913/1101224‑1101487                                                                                                                                   |                                                                                                                                      aCCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTgcg                                                                                                                              >  2:84460/1‑140 (MQ=255)      gCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGt                                                                                                                         >  1:357451/1‑140 (MQ=255)          gATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAg                                                                                                                     <  1:84460/140‑1 (MQ=255)           aTTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGc                                                                                                                    >  1:264568/1‑140 (MQ=255)                             aaCCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTgg                                                                                                  <  1:138453/140‑1 (MQ=255)                                            cTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGtata                                                                                   >  1:242267/1‑140 (MQ=255)                                              tCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATaaa                                                                                 >  2:125927/1‑140 (MQ=255)                                                     tGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCga                                                                          <  1:125927/140‑1 (MQ=255)                                                                  cgTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTg                                                             >  1:243864/1‑140 (MQ=255)                                                                    ttAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGcc                                                           <  1:374885/140‑1 (MQ=255)                                                                            tGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAg                                                   >  2:105186/1‑140 (MQ=255)                                                                            tGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAg                                                   >  2:186112/1‑140 (MQ=255)                                                                                 cGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTg                                              <  1:324364/140‑1 (MQ=255)                                                                                                 aTGGCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCgg                              <  2:264568/140‑1 (MQ=255)                                                                                                   ggCAACCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGaa                            <  1:186112/140‑1 (MQ=255)                                                                                                      aaCCGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAAcag                         <  2:299663/140‑1 (MQ=255)                                                                                                        ccGTGCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAAcagca                       >  1:388682/1‑140 (MQ=255)                                                                                                            gCCGTTTTCTTGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTgg                   <  2:120412/140‑1 (MQ=255)                                                                                                                     ttGTGCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGCCCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTAc          <  1:28302/140‑1 (MQ=255)                                                                                                                         gCCGCACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGc      <  2:388682/140‑1 (MQ=255)                                                                                                                             cACGAACAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCggg  >  1:38800/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                      ACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGCACGAATAATCTTGCGCTCGTTAAGCAGGTTTTGTAAGCCCTGGCGCTCCAGCGGTATAAACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGG  >  NC_000913/1101224‑1101487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑