Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,601	A→G	Y77H (TAT→CAT)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,601	0	A	G	100.0%	97.9 / NA	27	Y77H (TAT→CAT)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

ATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTG  >  NC_000913/1182464‑1182734                                                                                                                                          |                                                                                                                                      aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATggg                                                                                                                                     >  1:221227/1‑140 (MQ=255)   tCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCa                                                                                                                                   >  2:133198/1‑140 (MQ=255)     gggTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCAcc                                                                                                                                 <  2:141224/140‑1 (MQ=255)       gTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGt                                                                                                                               >  1:85729/1‑140 (MQ=255)            aaGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTg                                                                                                                          >  2:140994/1‑140 (MQ=255)                      gAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCa                                                                                                                <  1:5381/140‑1 (MQ=255)                                   tCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGc                                                                                                              <  1:12644/129‑1 (MQ=255)                                   tCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGc                                                                                                              >  2:12644/1‑129 (MQ=255)                                             tGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTttcgttc                                                                                         <  2:85729/140‑1 (MQ=255)                                                                 ttGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGAt                                                                     <  1:382286/140‑1 (MQ=255)                                                                 ttGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATAATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGAt                                                                     <  2:316920/140‑1 (MQ=255)                                                                                  catcCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt                                                    <  2:221227/140‑1 (MQ=255)                                                                                  catcCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt                                                    <  2:236788/140‑1 (MQ=255)                                                                                    tcCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt                                                    <  1:347710/138‑1 (MQ=255)                                                                                    tcCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt                                                    >  2:347710/1‑138 (MQ=255)                                                                                    tcCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCt                                                  >  2:74460/1‑140 (MQ=255)                                                                                         tcttTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGt                                             >  2:359576/1‑140 (MQ=255)                                                                                           tttACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGa                                           <  1:140994/140‑1 (MQ=255)                                                                                           tttACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGa                                           <  1:74460/140‑1 (MQ=255)                                                                                             tACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAAc                                         >  1:352746/1‑140 (MQ=255)                                                                                                          aaCGTAATAGGTTGAAGGAACCACCAGATCATGGGCACAGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTc                            <  2:166286/140‑1 (MQ=255)                                                                                                              tAATAGGTTGAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTgg                        >  2:187299/1‑140 (MQ=255)                                                                                                                       gAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTa               >  2:50288/1‑140 (MQ=255)                                                                                                                       gAAGGAACCACCAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTa               >  1:311637/1‑140 (MQ=255)                                                                                                                             accaccAGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGt         <  1:50288/140‑1 (MQ=255)                                                                                                                               caccaGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTc        <  1:53328/139‑1 (MQ=255)                                                                                                                               caccaGATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTc        >  2:53328/1‑139 (MQ=255)                                                                                                                                    gATCATGGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTg  <  1:187299/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      ATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTG  >  NC_000913/1182464‑1182734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑