Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,911	A→G	A387A (GCT→GCC)	cvrA ←	putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,911	0	A	G	100.0%	43.6 / NA	13	A387A (GCT→GCC)	cvrA	putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

CGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTG  >  NC_000913/1240774‑1241035                                                                                                                                          |                                                                                                                             cgcAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGTGGAACCACCACTTTGGCTTTTTTGgc                                                                                                                            >  1:203622/1‑140 (MQ=255)   cAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGgccg                                                                                                                          <  2:52620/140‑1 (MQ=255)     gTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGgccgcc                                                                                                                        <  2:122932/140‑1 (MQ=255)          gcgcCCACGCACCATTTATTGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACga                                                                                                                   <  1:115335/140‑1 (MQ=255)                      cATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTcc                                                                                                       >  1:417350/1‑140 (MQ=255)                        tttATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCt                                                                                                     >  1:112631/1‑140 (MQ=255)                                  cTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAg                                                                                           >  2:288308/1‑140 (MQ=255)                                      aTTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGaa                                                                                       <  2:417350/140‑1 (MQ=255)                                                         ccACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAgg                                                                    <  2:112631/140‑1 (MQ=255)                                                            cGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGa                                                                 <  1:210190/140‑1 (MQ=255)                                                                                         cgcgTGACACCGGACGTCCCACTGGTGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGc                                    <  2:203622/140‑1 (MQ=255)                                                                                                          cccACTGGCGGAACCACCACTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGaa                   >  1:216852/1‑140 (MQ=255)                                                                                                                           cacTTTGGCTTTTTTGGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTg  >  1:409652/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                             CGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTG  >  NC_000913/1240774‑1241035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑