Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,390	C→T	V154I (GTA→ATA)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,390	0	C	T	100.0%	48.3 / NA	15	V154I (GTA→ATA)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/10);  total (5/10)

AACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCC  >  NC_000913/1271260‑1271500                                                                                                                                   |                                                                                                                aacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGc                                                                                                        <  1:325947/140‑1 (MQ=255) aacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGc                                                                                                        <  2:147013/140‑1 (MQ=255) aacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGc                                                                                                        <  2:98411/140‑1 (MQ=255)            aTGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCA‑ggggggaac                                                                                            >  1:94130/1‑136 (MQ=255)              gCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAg                                                                                           <  1:336692/140‑1 (MQ=255)                gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAgcg                                                                                         <  1:394136/140‑1 (MQ=255)                gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAgcg                                                                                         <  2:125144/140‑1 (MQ=255)                          ggATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATa                                                                               <  1:68921/140‑1 (MQ=255)                                                                           tAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCg                              >  1:162125/1‑140 (MQ=255)                                                                                 cTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCcaaca                       <  2:67640/140‑1 (MQ=255)                                                                                  tGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCcaaca                       >  2:328595/1‑140 (MQ=255)                                                                                    aCCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACaat                     >  2:314096/1‑140 (MQ=255)                                                                                         ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGa                >  1:277722/1‑140 (MQ=255)                                                                                                  tCGCCGCAGGCAGAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc       <  2:277722/140‑1 (MQ=255)                                                                                                       gcaggcagAGCCATTGGAAATACCAGTATGATTATCGCCAGGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGAGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGcc  <  1:139046/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                AACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCA‑GGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCC  >  NC_000913/1271260‑1271500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑