Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,377,427	G→A	G199D (GGC→GAC)	ycjS →	putative oxidoreductase YcjS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,377,427	0	G	A	100.0%	65.5 / NA	20	G199D (GGC→GAC)	ycjS	putative oxidoreductase YcjS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

CGTTACCACCGCCCGCGCCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGGCTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGTTTGGCACCA  >  NC_000913/1377293‑1377552                                                                                                                                       |                                                                                                                              cGTTACCACCGCCCGCGCCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTc                                                                                                                          <  2:139304/140‑1 (MQ=255)      ccaccGCCCGCGCCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCcggcg                                                                                                                     >  2:266943/1‑140 (MQ=255)            cccgcGCCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGaaa                                                                                                               >  2:247438/1‑140 (MQ=255)                cgcCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCg                                                                                                           >  2:372538/1‑140 (MQ=255)                   ccTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGa                                                                                                        <  1:247438/140‑1 (MQ=255)                                 gcgTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCttt                                                                                          >  1:387066/1‑140 (MQ=255)                                            tGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCgg                                                                               >  1:418259/1‑140 (MQ=255)                                                             tAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCtgtg                                                              >  1:157658/1‑140 (MQ=255)                                                                      gCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTg                                                     <  2:387066/140‑1 (MQ=255)                                                                       cAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTgg                                                    >  2:341313/1‑140 (MQ=255)                                                                        aGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGt                                                   <  2:418259/140‑1 (MQ=255)                                                                          ggtggtGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGa                                                 >  2:52988/1‑140 (MQ=255)                                                                           gtggtgGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAg                                                <  1:52988/140‑1 (MQ=255)                                                                                ggCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGa                                           <  1:372538/140‑1 (MQ=255)                                                                                    cGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCg                                       <  1:22453/140‑1 (MQ=255)                                                                                              cATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACa                             <  2:157658/140‑1 (MQ=255)                                                                                                                 gATGCTGCGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTAAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGtt          >  1:255417/1‑140 (MQ=255)                                                                                                                      tgcGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGTTTGGCa     >  1:331289/1‑140 (MQ=255)                                                                                                                      tgcGATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGTTTGGCa     >  2:364066/1‑140 (MQ=255)                                                                                                                         gATGTATGTGCTGGACTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGTTTGGCACCa  >  1:14534/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                              CGTTACCACCGCCCGCGCCCTGCGTCGCTGCGGCGTTCCCGGCTGGGGTGTCTTTACCAATAAAGAACTGCAGGGTGGTGGCCCGCTGATCGACATCGGCATTCATATGCTGGATGCTGCGATGTATGTGCTGGGCTTTCCGGCGGTGAAAAGCGTGAATGCGCATAGCTTTCAAAAGATCGGCACGCAAAAGAGCTGTGGTCAATTTGGTGAGTGGGATCCGGCAACTTACAGCGTCGAAGATTCGCTGTTTGGCACCA  >  NC_000913/1377293‑1377552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑