Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	137,719	A→G	T213A (ACG→GCG)	cueO →	cuprous oxidase/multicopper oxidase with role in copper homeostasis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	137,719	0	A	G	100.0%	89.4 / NA	25	T213A (ACG→GCG)	cueO	cuprous oxidase/multicopper oxidase with role in copper homeostasis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

TAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCG  >  NC_000913/137591‑137841                                                                                                                                 |                                                                                                                           tAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGAcc                                                                                                                 <  1:146393/140‑1 (MQ=255)   aTGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCaa                                                                                                               <  2:220752/140‑1 (MQ=255)   aTGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCaa                                                                                                               <  1:238016/140‑1 (MQ=255)            aaaCAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAAt                                                                                                      <  1:36987/140‑1 (MQ=255)                  tGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACgg                                                                                                            >  2:328721/1‑128 (MQ=255)                  tGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACgg                                                                                                            <  1:328721/128‑1 (MQ=255)                                       gTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTgg                                                                           <  2:89872/140‑1 (MQ=255)                                              ttCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACTAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTg                                                                    >  1:59865/1‑140 (MQ=255)                                                             ttAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTc                                                     >  2:349790/1‑140 (MQ=255)                                                                 cgcCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATg                                                 >  1:390211/1‑140 (MQ=255)                                                                 cgcCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATg                                                 >  2:170719/1‑140 (MQ=255)                                                                   ccGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGc                                               <  1:170719/140‑1 (MQ=255)                                                                      aCGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATg                                                 <  1:322651/135‑1 (MQ=255)                                                                      aCGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATg                                                 >  2:322651/1‑135 (MQ=255)                                                                                        aaCTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAAt                          >  1:45163/1‑140 (MQ=255)                                                                                        aaCTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAAt                          >  2:110495/1‑140 (MQ=255)                                                                                        aaCTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAAt                          >  2:82652/1‑140 (MQ=255)                                                                                            ggATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCg                      <  1:110495/140‑1 (MQ=255)                                                                                             gATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCt                              <  1:280243/131‑1 (MQ=255)                                                                                             gATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCt                              >  2:280243/1‑131 (MQ=255)                                                                                                  gatgaCCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCt                              <  2:99966/126‑1 (MQ=255)                                                                                                  gatgaCCGCCGCCGTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCt                              >  1:99966/1‑126 (MQ=255)                                                                                                       ccgccgccgTGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGAc           >  2:294318/1‑140 (MQ=255)                                                                                                                tGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCg  <  2:204243/140‑1 (MQ=255)                                                                                                                tGGGCTGGTTTGGCGATGCGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCg  <  1:353850/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                           TAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCAACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCG  >  NC_000913/137591‑137841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑