Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,657,835	T→C	S91P (TCT→CCT)	ynfD →	DUF1161 domain‑containing protein YnfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,657,835	0	T	C	100.0%	104.3 / NA	29	S91P (TCT→CCT)	ynfD	DUF1161 domain‑containing protein YnfD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

ACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAT  >  NC_000913/1657720‑1657966                                                                                                                    |                                                                                                                                    aCCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCg                                                                                                             <  2:315574/140‑1 (MQ=255)    cAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAAc                                                                                                          <  1:413954/140‑1 (MQ=255)     aaTGACCAGGTTGATTAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAAc                                                                                                          <  2:376004/139‑1 (MQ=255)     aaTGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAAc                                                                                                          >  1:376004/1‑139 (MQ=255)       tGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGc                                                                                                       >  1:28710/1‑140 (MQ=255)             ggTTGATCCGGCTTATTCCCAGGTCGTCGGCCCTTGCGCTAATGAATCCCCTAAAATTCTCTAATCCCCCCCCCCCAGCGGTAACGTCTCTTCCCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAac                                                                                                 <  2:376876/140‑1 (MQ=255)                  aTCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAAc                                                                                                          >  1:117011/1‑126 (MQ=255)                  aTCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAAc                                                                                                          <  2:117011/126‑1 (MQ=255)                   tCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTa                                                                                           >  2:55359/1‑140 (MQ=255)                         tGATCCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACt                                                                                     <  1:343751/140‑1 (MQ=255)                              cccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACg                                                                                <  2:127938/140‑1 (MQ=255)                                      gtcgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat                                                                        >  1:411146/1‑140 (MQ=255)                                              ttGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATc                                                                >  1:87058/1‑140 (MQ=255)                                                    tAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGa                                                          <  1:4843/140‑1 (MQ=255)                                                            cGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGa                                                     >  1:97799/1‑137 (MQ=255)                                                            cGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGa                                                     <  2:97799/137‑1 (MQ=255)                                                               aTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCTCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATac                                               <  2:291804/140‑1 (MQ=255)                                                                             aCCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCa                                   >  1:369770/1‑138 (MQ=255)                                                                             aCCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCa                                   <  2:369770/138‑1 (MQ=255)                                                                                  caccacCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt                            >  2:118088/1‑140 (MQ=255)                                                                                  caccacCAGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt                            >  2:53360/1‑140 (MQ=255)                                                                                       ccaGCGGTAACGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCa                       <  1:311149/140‑1 (MQ=255)                                                                                                 cGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCtta                              <  2:159402/123‑1 (MQ=255)                                                                                                 cGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCtta                              >  1:159402/1‑123 (MQ=255)                                                                                                 cGTCTCTGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTa             <  2:28710/140‑1 (MQ=255)                                                                                                     tctGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGa         >  1:54307/1‑140 (MQ=255)                                                                                                     tctGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGa         <  1:118088/140‑1 (MQ=255)                                                                                                      ctGCTCCCGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGat        <  1:392168/140‑1 (MQ=255)                                                                                                            ccGCGCAGCCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAt  >  1:115868/1‑140 (MQ=255)                                                                                                                    |                                                                                                                                    ACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAT  >  NC_000913/1657720‑1657966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑