Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,961	C→T	A202T (GCA→ACA)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,961	0	C	T	100.0%	100.5 / NA	30	A202T (GCA→ACA)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

TGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACC  >  NC_000913/1667825‑1668081                                                                                                                                         |                                                                                                                         tgGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCAt                                                                                                                       <  1:222415/140‑1 (MQ=255)   gCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCg                                                                                                                     >  1:196042/1‑140 (MQ=255)    ccTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGt                                                                                                                    >  2:408410/1‑140 (MQ=255)      tATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTcc                                                                                                                  <  1:322169/140‑1 (MQ=255)       aTTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCa                                                                                                                 >  2:337240/1‑140 (MQ=255)       aTTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCa                                                                                                                 <  1:393152/140‑1 (MQ=255)            cACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATgcgc                                                                                                            <  2:196042/140‑1 (MQ=255)                  actactGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGatat                                                                                                      >  1:50600/1‑140 (MQ=255)                            cctgcGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAat                                                                                            >  2:31837/1‑140 (MQ=255)                                       cAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGaa                                                                                 <  1:224488/140‑1 (MQ=255)                                       cAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGaa                                                                                 <  1:408410/140‑1 (MQ=255)                                          aTGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGc                                                                              >  2:9419/1‑140 (MQ=255)                                                aTCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAt                                                                        <  2:50600/140‑1 (MQ=255)                                                            gcccgcccCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAgcg                                                            >  2:85359/1‑140 (MQ=255)                                                                      aCGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAg                                                  >  1:339246/1‑140 (MQ=255)                                                                       cGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGc                                                 <  1:280626/140‑1 (MQ=255)                                                                            tgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCAt                                            >  1:157878/1‑140 (MQ=255)                                                                             gATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCAtc                                           <  2:320459/140‑1 (MQ=255)                                                                                      ccaccTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACa                                  <  2:398075/140‑1 (MQ=255)                                                                                         ccTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATcc                               >  1:366499/1‑140 (MQ=255)                                                                                          cTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCAtc                                           <  2:218056/127‑1 (MQ=255)                                                                                          cTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCAtc                                           >  1:218056/1‑127 (MQ=255)                                                                                             aaTCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGt                           >  1:398361/1‑140 (MQ=255)                                                                                                     cgcgcgCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGc                   <  1:85359/140‑1 (MQ=255)                                                                                                     cgcgcgCCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGc                   <  2:398361/140‑1 (MQ=255)                                                                                                        gcgcCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATg                <  2:366499/140‑1 (MQ=255)                                                                                                          gcCCCGCGTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCg              <  1:348837/140‑1 (MQ=255)                                                                                                                cgTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCg              <  2:106153/134‑1 (MQ=255)                                                                                                                cgTGAGGCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCg              >  1:106153/1‑134 (MQ=255)                                                                                                                      gCACCAAACAAGGCCTCTGTCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATAcc  >  2:352975/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                         TGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACC  >  NC_000913/1667825‑1668081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑