Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,802,766	T→C	intergenic (‑196/‑328)	thrS ← / → arpB	threonine‑‑tRNA ligase/putative ankyrin repeat protein B, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,802,766	0	T	C	100.0%	60.7 / NA	18	intergenic (‑196/‑328)	thrS/arpB	threonine‑‑tRNA ligase/putative ankyrin repeat protein B, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

ATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGTACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAG  >  NC_000913/1802633‑1802890                                                                                                                                      |                                                                                                                             attaACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCttt                                                                                                                        <  2:16088/140‑1 (MQ=255)                cGAAACCATCTAGCCAACAAATGCTAAATTGGCTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTgat                                                                                                         >  2:371655/1‑140 (MQ=255)                        tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGattcgattcg                                                                                                 >  2:256501/1‑140 (MQ=255)                                 caaATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGtt                                                                                        >  2:383733/1‑140 (MQ=255)                                  aaaTGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGttt                                                                                       <  2:303859/140‑1 (MQ=255)                                     tGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTa                                                                                    <  1:383733/140‑1 (MQ=255)                                          aaTTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAg                                                                               <  1:256501/140‑1 (MQ=255)                                                           gTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCt                                                              <  2:316919/140‑1 (MQ=255)                                                                     gAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGaacaa                                                    <  2:4809/140‑1 (MQ=255)                                                                        aGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTg                                                 >  2:402633/1‑140 (MQ=255)                                                                         gACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGa                                                <  2:263374/140‑1 (MQ=255)                                                                                 ccTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCa                                        <  1:236846/140‑1 (MQ=255)                                                                                               cccGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGaaa                          >  2:311568/1‑140 (MQ=255)                                                                                                 cGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAgg                        >  2:218355/1‑140 (MQ=255)                                                                                                   gTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGtt                      >  2:308542/1‑140 (MQ=255)                                                                                                             gTTAACACTAAAAAAAGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGc            <  1:311568/140‑1 (MQ=255)                                                                                                                      aaaaaaaGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTAca   >  1:379441/1‑140 (MQ=255)                                                                                                                       aaaaaaGGGAGATTGCACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTAcag  <  1:218355/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                             ATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCCGGTTGAGCGATGTTAACACTAAAAAAAGGGAGATTGTACCTTTCCGTTTCACATTGATTCGATTCGCAATTCGTTTGTAGTGAGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAG  >  NC_000913/1802633‑1802890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑