Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,857,011	T→C	E253E (GAA→GAG)	ydjI ←	putative aldolase YdjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,857,011	0	T	C	100.0%	108.7 / NA	30	E253E (GAA→GAG)	ydjI	putative aldolase YdjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

ATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGAT  >  NC_000913/1856882‑1857146                                                                                                                                  |                                                                                                                                        atatCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACCTCACGCTCTAAATGCa                                                                                                                               >  1:21738/1‑140 (MQ=255)    tCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGa                                                                                                                            <  1:155752/140‑1 (MQ=255)                gCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCt                                                                                                                >  1:317998/1‑140 (MQ=255)                  aCCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTgg                                                                                                              <  1:364957/140‑1 (MQ=255)                          aaaTAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTtttcttt                                                                                                      <  2:359907/140‑1 (MQ=255)                              aaCGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACt                                                                                                  >  1:264724/1‑140 (MQ=255)                                          aTCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCg                                                                                      <  2:317998/140‑1 (MQ=255)                                           tCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTtttctt                                                                                                       <  1:187701/122‑1 (MQ=255)                                           tCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTtttctt                                                                                                       >  2:187701/1‑122 (MQ=255)                                                atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCAtt                                                                                         <  2:325703/131‑1 (MQ=255)                                                atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCAtt                                                                                         >  1:325703/1‑131 (MQ=255)                                                atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTg                                                                                        <  2:37764/132‑1 (MQ=255)                                                atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTg                                                                                        >  1:37764/1‑132 (MQ=255)                                                atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGa                                                                                >  2:28072/1‑140 (MQ=255)                                                   attattCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACag                                                                             <  1:28072/140‑1 (MQ=255)                                                    ttattCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACaga                                                                            <  1:18764/140‑1 (MQ=255)                                                     tattCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACagag                                                                           >  2:182288/1‑140 (MQ=255)                                                            cTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTa                                                                    >  2:205245/1‑140 (MQ=255)                                                               tGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGa                                                                 >  2:20225/1‑140 (MQ=255)                                                                                                        tCTTTTACAGCTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAAt                        <  1:20225/140‑1 (MQ=255)                                                                                                                  cTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCa              <  2:264724/140‑1 (MQ=255)                                                                                                                  cTTTACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCa              >  2:305489/1‑140 (MQ=255)                                                                                                                    ttACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCgg                   <  1:311245/133‑1 (MQ=255)                                                                                                                    ttACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCgg                   >  2:311245/1‑133 (MQ=255)                                                                                                                    ttACGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACt            >  1:12391/1‑140 (MQ=255)                                                                                                                       cGTACTTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGAt         <  1:305489/140‑1 (MQ=255)                                                                                                                       cGTACCTCACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGAt         <  2:21738/140‑1 (MQ=255)                                                                                                                              cACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCAt                <  1:210441/126‑1 (MQ=255)                                                                                                                              cACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCAt                >  2:210441/1‑126 (MQ=255)                                                                                                                              cACGCTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGAt  >  2:182551/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        ATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGAT  >  NC_000913/1856882‑1857146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑