Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,884,146	T→C	V320A (GTA→GCA)	yeaV →	putative transporter YeaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,884,146	0	T	C	100.0%	56.9 / NA	17	V320A (GTA→GCA)	yeaV	putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

CGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCTCGCA  >  NC_000913/1884033‑1884278                                                                                                                  |                                                                                                                                     cgGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGaaa                                                                                                            >  1:43269/1‑140 (MQ=255)           aTTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGggcgg                                                                                                  <  1:185881/140‑1 (MQ=255)                      tATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                                                       >  1:248852/1‑140 (MQ=255)                            gggACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGc                                                                                 >  2:97239/1‑140 (MQ=255)                                  gATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                                                           >  1:3428/1‑140 (MQ=255)                                              caCCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGc                                                               >  1:160869/1‑140 (MQ=255)                                                   tCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCTCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGcc                                                          <  2:369125/140‑1 (MQ=255)                                                                 ttcttTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCt                                            <  2:160869/140‑1 (MQ=255)                                                                      tGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCt                                            <  1:101760/135‑1 (MQ=255)                                                                      tGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCt                                            >  2:101760/1‑135 (MQ=255)                                                                                        tGCCATTCATCAGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGtgatga                     >  1:125009/1‑140 (MQ=255)                                                                                               catcaGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTAtttt              >  1:148510/1‑140 (MQ=255)                                                                                                 tcaGTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTcc            <  2:269524/140‑1 (MQ=255)                                                                                                    gTTTATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTg         <  1:164437/140‑1 (MQ=255)                                                                                                       tATCAATGGTGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCt      <  2:58704/140‑1 (MQ=255)                                                                                                       tATCAATGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCt      <  1:154956/140‑1 (MQ=255)                                                                                                           aaTGGCGCAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCTCGCa  <  2:43269/140‑1 (MQ=255)                                                                                                               gcgcAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCt      <  1:279262/132‑1 (MQ=255)                                                                                                                  |                                                                                                                                     CGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCATACCTGGGCGTGATGATTATTTTCCTTGCCTCGCA  >  NC_000913/1884033‑1884278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑