Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,417	T→C	V56A (GTT→GCT)	yebB →	putative papain‑like amidase YebB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,417	0	T	C	100.0%	86.1 / NA	24	V56A (GTT→GCT)	yebB	putative papain‑like amidase YebB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

CTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGTTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGACGCTGGGCTGACAGAACGACAAAAACAACGAATTGTTGAAC  >  NC_000913/1946295‑1946536                                                                                                                           |                                                                                                                        cTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATca                                                                                                        <  2:263664/140‑1 (MQ=255)      cATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACg                                                                                                   <  2:314793/140‑1 (MQ=255)         gTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTa                                                                                                >  1:110150/1‑140 (MQ=255)           ataGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTAtc                                                                                              >  2:223359/1‑140 (MQ=255)                  ctgcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATcacc                                                                                                      <  2:397068/125‑1 (MQ=255)                  ctgcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATcacc                                                                                                      >  1:397068/1‑125 (MQ=255)                  ctgcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGtttt                                                                                       >  2:322193/1‑140 (MQ=255)                  ctgcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGtttt                                                                                       >  1:398169/1‑140 (MQ=255)                  ctgcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGtttt                                                                                       >  1:79149/1‑140 (MQ=255)                  ctgcCCTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGtttt                                                                                       >  2:39549/1‑140 (MQ=255)                    gcCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTttat                                                                                     >  1:58264/1‑140 (MQ=255)                          tttGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACg                                                                               <  2:194646/140‑1 (MQ=255)                               tCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGt                                                                          >  1:261299/1‑140 (MQ=255)                                    tatCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAAt                                                                     <  1:322193/140‑1 (MQ=255)                                         gCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACg                                                                <  2:110150/140‑1 (MQ=255)                                         gCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACg                                                                <  1:331129/140‑1 (MQ=255)                                           tGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACgct                                                              <  2:79149/140‑1 (MQ=255)                                             cATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACgctat                                                            >  1:336857/1‑140 (MQ=255)                                               tCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACgctatgc                                                          >  2:413687/1‑140 (MQ=255)                                                  aaTTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACgctatgctat                                                       <  2:58264/140‑1 (MQ=255)                                                               aTCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGAc                                          <  2:398169/140‑1 (MQ=255)                                                                 cACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGTTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGACGc                                        <  1:322047/140‑1 (MQ=255)                                                                  aCGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGACGCt                                       <  1:413687/140‑1 (MQ=255)                                                                                                       ccTGGTTGCAGAAAGCCGGGCTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGACGCTGGGCTGACAGAACGACAAAAACAACGAATTGTTGAAc  >  2:318667/1‑140 (MQ=255)                                                                                                                           |                                                                                                                        CTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTGAAGACTTCCTGGTTGCAGAAAGCCGGGTTCCCCTTTCAACCATCACCACGCTATCTCGTTTTATTAAACGTTCGTCTAATCAACGCTATGCTATAAAGCGATTAGACGCTGGGCTGACAGAACGACAAAAACAACGAATTGTTGAAC  >  NC_000913/1946295‑1946536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑