Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,984,599	C→T	A157A (GCG→GCA)	araG ←	arabinose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,984,599	0	C	T	100.0%	73.3 / NA	23	A157A (GCG→GCA)	araG	arabinose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

ATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAAACCCGCCTCATAAT  >  NC_000913/1984462‑1984708                                                                                                                                          |                                                                                                              aTACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAgtgc                                                                                                             >  1:324466/1‑140 (MQ=255)       tGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGc                                                                                                       >  2:314520/1‑140 (MQ=255)       tGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGc                                                                                                       <  1:267368/140‑1 (MQ=255)               gTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCaa                                                                                               <  2:412386/140‑1 (MQ=255)               gTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCaa                                                                                               >  2:388906/1‑140 (MQ=255)               gTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCaa                                                                                               >  1:250425/1‑140 (MQ=255)               gTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCaa                                                                                               <  2:244970/140‑1 (MQ=255)                         aCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCa                                                                                     >  2:68439/1‑140 (MQ=255)                          cccGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTACCAc                                                                                    >  1:277523/1‑140 (MQ=255)                              ccccTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGAc                                                                                <  2:124616/140‑1 (MQ=255)                                         gCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGgagaga                                                                     <  2:229732/140‑1 (MQ=255)                                           aGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGgagaga                                                                     <  2:265015/138‑1 (MQ=255)                                           aGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGgagaga                                                                     >  1:265015/1‑138 (MQ=255)                                             ttCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATAtt                                                                 >  1:238125/1‑140 (MQ=255)                                                          cgGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTc                                                    <  2:250425/140‑1 (MQ=255)                                                                 gATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCa                                             <  1:388906/140‑1 (MQ=255)                                                                 gATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCa                                             <  1:68439/140‑1 (MQ=255)                                                                 gATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCa                                             <  2:324466/140‑1 (MQ=255)                                                                 gATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCa                                             <  2:406867/140‑1 (MQ=255)                                                                              cagggCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCaa                                <  1:133090/138‑1 (MQ=255)                                                                                      agagGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTa                        <  1:143960/140‑1 (MQ=255)                                                                                            aGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGGCCGGGGCAATATTCATACCAAGATTGTTAAGtctg                  >  2:133090/1‑137 (MQ=255)                                                                                                 gTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAAAcc             >  1:216691/1‑140 (MQ=255)                                                                                                            aaaGGCGATAATTTTGGCGTTACGCGCCAGTGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAAACCCGCCTCATAAt  <  1:314520/140‑1 (MQ=255)                                                                                                                                          |                                                                                                              ATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAAACCCGCCTCATAAT  >  NC_000913/1984462‑1984708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑