Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,991,910	A→G	intergenic (‑9/+4)	leuZ ← / ← cysT	tRNA‑Leu/tRNA‑Cys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,991,910	0	A	G	100.0%	64.0 / NA	18	intergenic (‑9/+4)	leuZ/cysT	tRNA‑Leu/tRNA‑Cys
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

ACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAA  >  NC_000913/1991778‑1992040                                                                                                                                     |                                                                                                                                   acTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGa                                                                                                                             >  2:49909/1‑140 (MQ=255)     cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCAGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGgcg                                                                                                                         >  1:310786/1‑140 (MQ=255)               tGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAg                                                                                                               >  1:357533/1‑140 (MQ=255)                gATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGt                                                                                                              <  1:243120/140‑1 (MQ=255)                    ttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGaa                                                                                                          <  1:357849/140‑1 (MQ=255)                           tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACt                                                                                                   >  1:89936/1‑140 (MQ=255)                              cTTTCCCATGGTACCCGGAGCAGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                                                <  2:310786/140‑1 (MQ=255)                                      tGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTg                                                                                        <  2:374504/140‑1 (MQ=255)                                        gTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                                                      <  2:89936/140‑1 (MQ=255)                                           cccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATc                                                                                   <  2:357533/140‑1 (MQ=255)                                                aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                                                      <  2:281283/132‑1 (MQ=255)                                                aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                                                      >  1:281283/1‑132 (MQ=255)                                                                                             tCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGcc                                 >  1:45591/1‑140 (MQ=255)                                                                                                       cTACCGATTCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGc                       >  1:196863/1‑140 (MQ=255)                                                                                                              ttCCACCATCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGc                >  2:205855/1‑140 (MQ=255)                                                                                                                   ccaTCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATg           <  2:196863/140‑1 (MQ=255)                                                                                                                    caTCCGGGCTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGt          >  1:6803/1‑140 (MQ=255)                                                                                                                            cTCGGGAAGGAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTaaa  <  2:6803/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAA  >  NC_000913/1991778‑1992040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑