| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,017,557 | A→G | Q335R (CAG→CGG) | fliI → | flagellum‑specific ATP synthase FliI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,017,557 | 0 | A | G | 94.1% | 52.0 / ‑3.8 | 17 | Q335R (CAG→CGG) | fliI | flagellum‑specific ATP synthase FliI |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (6/10); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
ACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCG > NC_000913/2017447‑2017676 | aCCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCggg < 1:285838/140‑1 (MQ=255) cGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCg < 1:112402/140‑1 (MQ=255) gTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCggg < 2:401625/137‑1 (MQ=255) gTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCggg > 1:401625/1‑137 (MQ=255) gTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGa > 1:17810/1‑140 (MQ=255) tttGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTCCCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGa < 2:164000/140‑1 (MQ=255) tttGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGa < 2:17810/140‑1 (MQ=255) ccAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGt < 2:120635/140‑1 (MQ=255) gCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTc > 2:216025/1‑123 (MQ=255) gCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTc < 1:216025/123‑1 (MQ=255) gTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCg > 1:219422/1‑140 (MQ=255) gTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCA‑GATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGc > 1:97861/1‑140 (MQ=255) gTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCg > 2:169759/1‑140 (MQ=255) gCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCggg < 1:260194/140‑1 (MQ=255) cATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGc > 1:24299/1‑140 (MQ=255) cggcggcTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCgg < 2:219422/140‑1 (MQ=255) ccGTGCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGc < 1:218693/140‑1 (MQ=255) gCTCACTGAAGGCGATGACCGGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCcgcg < 2:201770/140‑1 (MQ=255) | ACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCG > NC_000913/2017447‑2017676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |