Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,031,989	C→T	C110Y (TGC→TAC)	dcm ←	DNA‑cytosine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,031,989	0	C	T	100.0%	75.5 / NA	23	C110Y (TGC→TAC)	dcm	DNA‑cytosine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/9);  total (14/9)

CCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAAC  >  NC_000913/2031866‑2032117                                                                                                                            |                                                                                                                                 ccTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGa                                                                                                                  >  1:257287/1‑140 (MQ=255)     cttTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCa                                                                                                              >  2:64188/1‑140 (MQ=255)              tGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGa                                                                                                     <  1:361197/140‑1 (MQ=255)                      aTGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATgcc                                                                                             >  2:121160/1‑140 (MQ=255)                                 atCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCgg                                                                                  >  1:67581/1‑140 (MQ=255)                                    ttCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGa                                                                               >  1:53532/1‑140 (MQ=255)                                     tCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGaa                                                                              <  1:121160/140‑1 (MQ=255)                                     tCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGaa                                                                              <  2:53532/140‑1 (MQ=255)                                         tAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTcc                                                                                    <  1:155019/130‑1 (MQ=255)                                         tAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTcc                                                                                    >  2:155019/1‑130 (MQ=255)                                             atgatgCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGa                                                                      >  2:335269/1‑140 (MQ=255)                                                 tgCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCg                                                                                >  1:304918/1‑126 (MQ=255)                                                 tgCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCg                                                                                <  2:304918/126‑1 (MQ=255)                                                             tCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTTCGGCGAATAGATCGATAAAGCGAAACGCATa                                                      >  2:320907/1‑140 (MQ=255)                                                                cAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATg                                                   >  2:417515/1‑140 (MQ=255)                                                                   taatGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCgg                                                >  2:195903/1‑140 (MQ=255)                                                                         ttGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGatgatg                                          <  1:417515/140‑1 (MQ=255)                                                                                     gTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGttt                              <  1:64188/140‑1 (MQ=255)                                                                                         gTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCggtg                                  <  1:301523/132‑1 (MQ=255)                                                                                         gTACCGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCggtg                                  >  2:301523/1‑132 (MQ=255)                                                                                            ccGCATGTTTGTTCCATTCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGtaat                       <  1:195903/140‑1 (MQ=255)                                                                                                             tCGCTGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGc      >  1:333570/1‑140 (MQ=255)                                                                                                                 tGGTAAACACGTACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAAc  >  1:252799/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                 CCTTCTTTATGGCTGAGGGTGATGTCGCGGATATCTTCATTAAAATGATGCGTCGCCGGATCGCAATAATGGTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAAC  >  NC_000913/2031866‑2032117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑