Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,063,300	G→A	C8C (TGC→TGT)	ldtA ←	L,D‑transpeptidase ErfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,063,300	0	G	A	100.0%	59.1 / NA	18	C8C (TGC→TGT)	ldtA	L,D‑transpeptidase ErfK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

CGGCTGGGTATTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAGCAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCGGTAGAACAATATTACT  >  NC_000913/2063177‑2063435                                                                                                                            |                                                                                                                                        cGGCTGGGTATTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTacac                                                                                                                         >  1:209169/1‑140 (MQ=255)    cTGGGTATTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACacgac                                                                                                                      <  2:391808/140‑1 (MQ=255)       ggTATTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGca                                                                                                                   <  2:209169/140‑1 (MQ=255)          aTTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGcatca                                                                                                                <  1:115947/140‑1 (MQ=255)            tgtgATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATg                                                                                                              <  2:119164/140‑1 (MQ=255)                           ttACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTc                                                                                               >  1:364730/1‑140 (MQ=255)                                     cGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTa                                                                                     >  2:65992/1‑140 (MQ=255)                                      gACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAATGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTAt                                                                                    <  2:380917/140‑1 (MQ=255)                                              cACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAg                                                                            >  2:21592/1‑140 (MQ=255)                                                                     gTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCg                                                     >  2:80005/1‑140 (MQ=255)                                                                                           gCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGaa                               >  2:381575/1‑140 (MQ=255)                                                                                               gTATGGCTGGCAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCg                           >  2:184029/1‑140 (MQ=255)                                                                                                     ctggcAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTa                         >  2:252043/1‑136 (MQ=255)                                                                                                     ctggcAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTa                         <  1:252043/136‑1 (MQ=255)                                                                                                         cAAAAAGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCgg                 <  1:381575/140‑1 (MQ=255)                                                                                                              aGCAGAGCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCGGTAGaa            >  2:201429/1‑140 (MQ=255)                                                                                                                  gagCAAATGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCGGTAGAACAat        >  1:366741/1‑140 (MQ=255)                                                                                                                        aTGAACAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCGGTAGAACAATATTACt  <  2:60204/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                        CGGCTGGGTATTGTGATCAGGAACAGTTACAGTAAACGACTGCCCCACTAAACGGCTACCCTCTGGAGGTAATGGATAAGTTACCGCCAGGCTAGTATGGCTGGCAAAAAGCAGAGCAAATGAGCAAAGAATATTTACACGACGCATCATGTCCCTTTCCTATGTCGCGAAAGCTATCCGTTAAGTATAGCTTTTATCAGACTTTTCGTTTTTAACTGTTCAAATCAGAAGTCGTATTCCCCGGTAGAACAATATTACT  >  NC_000913/2063177‑2063435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑