Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	230,227	A→G	S219P (TCA→CCA)	yafC ←	putative LysR family transcriptional regulator YafC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	230,227	0	A	G	100.0%	70.0 / NA	20	S219P (TCA→CCA)	yafC	putative LysR family transcriptional regulator YafC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/12);  total (8/12)

CTTTATCTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGACAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGATCGTTTCTGGCTTCCCG  >  NC_000913/230101‑230354                                                                                                                               |                                                                                                                                cTTTATCTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTc                                                                                                                    <  1:409281/140‑1 (MQ=255)    tATCTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCAcc                                                                                                                 <  2:308027/140‑1 (MQ=255)     aTCTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATAGCAAACCGTACTTCACCt                                                                                                                >  2:207382/1‑140 (MQ=255)       cTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCa                                                                                                              <  1:409039/140‑1 (MQ=255)                   ccaccaATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCa                                                                                                  <  1:72045/140‑1 (MQ=255)                               cgcgAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTa                                                                                            <  2:354997/134‑1 (MQ=255)                               cgcgAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTa                                                                                            >  1:354997/1‑134 (MQ=255)                                      gaTTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATAGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGcc                                                                               <  1:207382/140‑1 (MQ=255)                                      gaTTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCACTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGcc                                                                               <  1:44493/140‑1 (MQ=255)                                              tGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCg                                                                                  <  1:54984/129‑1 (MQ=255)                                              tGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCg                                                                                  >  2:54984/1‑129 (MQ=255)                                              tGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTg                                                                       >  2:338903/1‑140 (MQ=255)                                                          aGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCggg                                                            >  1:49616/1‑139 (MQ=255)                                                          aGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCggg                                                            <  2:49616/139‑1 (MQ=255)                                                              ggACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAg                                                       >  2:181137/1‑140 (MQ=255)                                                                   aaCACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAAt                                                  >  1:364192/1‑140 (MQ=255)                                                                                                  cTGTTTCAGTGTTTCCCCACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCGGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGa                   <  2:369034/140‑1 (MQ=255)                                                                                                                   cACTATTGGATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGATCGTTTCTGGCTTCCCg  <  1:338903/140‑1 (MQ=255)                                                                                                                           gATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGATCGTTTCTGGCTTcc    <  2:119762/130‑1 (MQ=255)                                                                                                                           gATGGCAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGATCGTTTCTGGCTTcc    >  1:119762/1‑130 (MQ=255)                                                                                                                               |                                                                                                                                CTTTATCTGCCATTAACTCCACCAATTCTCCGCGAGCGATTTCTTTGTCGATCATGTAGTCGGACAAACACGCAATCCCGTTGCCACTCAGGCAAAGCTGTTTCAGTGTTTCCCCACTATTGGATGACAAACCGTACTTCACCTCATGTAATTGTCCATCGCTACGGGCTATCGGCCAGGTATTGAGGGAAGCGGGTTCAGTGAATCCCAGGCAAATATGTTGCTTTAAATCGTCGATCGTTTCTGGCTTCCCG  >  NC_000913/230101‑230354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑