Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,087,981	T→C	N93S (AAC→AGC)	yeeY ←	putative DNA‑binding transcriptional regulator YeeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,087,981	0	T	C	100.0%	73.6 / NA	21	N93S (AAC→AGC)	yeeY	putative DNA‑binding transcriptional regulator YeeY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/8);  total (13/8)

GGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCG  >  NC_000913/2087847‑2088100                                                                                                                                       |                                                                                                                        gggCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTcc                                                                                                                    >  2:253579/1‑140 (MQ=255) gggCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTcc                                                                                                                    >  2:105153/1‑140 (MQ=255) gggCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACACCAATCACCAGCTCGCTTTcc                                                                                                                    >  2:7575/1‑140 (MQ=255)       cccATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCt                                                                                                              >  1:219521/1‑140 (MQ=255)          aTGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCNCTTTCCCAGCCTTCa                                                                                                           <  1:257551/140‑1 (MQ=255)                   cGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTg                                                                                                  >  2:300708/1‑140 (MQ=255)                            cATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGc                                                                                         >  1:270548/1‑140 (MQ=255)                                         cAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTccc                                                                            <  2:316657/140‑1 (MQ=255)                                                      cTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACACCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAg                                                               <  1:7575/140‑1 (MQ=255)                                                              ttGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCa                                                                         >  2:362619/1‑122 (MQ=255)                                                              ttGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCa                                                                         <  1:362619/122‑1 (MQ=255)                                                                     aaaGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCttt                                                >  2:401773/1‑140 (MQ=255)                                                                           tCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaa                                          >  2:203652/1‑140 (MQ=255)                                                                                      cgcAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTc                               >  1:141785/1‑140 (MQ=255)                                                                                           gCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTg                          >  2:249806/1‑140 (MQ=255)                                                                                                         aaCGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAgc            >  1:148167/1‑140 (MQ=255)                                                                                                         aaCGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAgc            >  2:63583/1‑140 (MQ=255)                                                                                                          aCGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAgcg           <  2:219521/140‑1 (MQ=255)                                                                                                          aCGTGTCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAgcg           <  1:249806/140‑1 (MQ=255)                                                                                                            gtgtCGTCCACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGcgcg         <  1:182655/140‑1 (MQ=255)                                                                                                                   ccACGCCAATCACCAGCTCGCTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCg  <  2:141785/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                        GGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCG  >  NC_000913/2087847‑2088100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑