Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,170,193	A→G	intergenic (‑26/‑43)	gatR ← / → insE‑5	DNA‑binding transcriptional repressor GatR, N‑terminal fragment/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,170,193	0	A	G	100.0%	24.6 / NA	8	intergenic (‑26/‑43)	gatR/insE‑5	DNA‑binding transcriptional repressor GatR, N‑terminal fragment/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

GCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATT  >  NC_000913/2170066‑2170281                                                                                                                                |                                                                                         gCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAg                                                                              <  2:17300/140‑1 (MQ=255)           ccGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTa                                                                                <  1:133509/128‑1 (MQ=255)           ccGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTa                                                                                >  2:133509/1‑128 (MQ=255)                       tGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAgg                                                        <  1:411660/140‑1 (MQ=255)                                   tGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGa                                            >  1:265392/1‑140 (MQ=255)                                                   cATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAc                            <  2:323856/140‑1 (MQ=255)                                                              cGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAAc                 >  1:352111/1‑140 (MQ=255)                                                                             tCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGGCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCAtt  >  2:212728/1‑140 (MQ=255)                                                                                                                                |                                                                                         GCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATT  >  NC_000913/2170066‑2170281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑