Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,485	G→A	R462R (CGG→CGA)	yehQ →	SWIM zinc finger domains‑containing protein YehQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,485	0	G	A	100.0%	75.7 / NA	23	R462R (CGG→CGA)	yehQ	SWIM zinc finger domains‑containing protein YehQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

CCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAAGATAATGTGCTGACGTT  >  NC_000913/2210355‑2210616                                                                                                                                   |                                                                                                                                    cccGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAt                                                                                                                            >  1:237201/1‑140 (MQ=255)           cTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATa                                                                                                                  >  2:335177/1‑140 (MQ=255)            ttAAGCGGCGTTGTGCCGCTGTCGCCTGATGCCTGGAAAATGTTGAGCGCGCCGTTACGCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATaa                                                                                                                 <  2:103696/140‑1 (MQ=255)                      ttGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTtatttatt                                                                                                       >  2:40243/1‑140 (MQ=255)                      ttGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTtatttatt                                                                                                       >  2:223358/1‑140 (MQ=255)                         tgCCGCTGTCGCCTGATGCCTGGAAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTg                                                                                                    <  2:392267/140‑1 (MQ=255)                            cGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                                                                 <  1:223358/140‑1 (MQ=255)                                 tCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGc                                                                                            <  2:148108/140‑1 (MQ=255)                                  cGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCt                                                                                           <  1:28953/140‑1 (MQ=255)                                           ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                                                                  >  1:26047/1‑140 (MQ=255)                                             tGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTc                                                                                >  2:213684/1‑140 (MQ=255)                                               gCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGc                                                                              <  1:213684/140‑1 (MQ=255)                                                                     cGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa                                                        <  1:251961/140‑1 (MQ=255)                                                                        cAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc                                                     >  1:131911/1‑140 (MQ=255)                                                                          gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGc                                                   >  2:105058/1‑140 (MQ=255)                                                                              ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa                                               <  2:26047/140‑1 (MQ=255)                                                                                     tgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGTCGCCAGACGCTGGATGCGCAg                                        >  2:360338/1‑140 (MQ=255)                                                                                      ggtttggCGGGAATTTTTCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAgg                                       <  2:131911/134‑1 (MQ=255)                                                                                                  atttttCCGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAgcggcg                           <  2:237201/137‑1 (MQ=255)                                                                                                       tACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAggg                      <  1:105058/140‑1 (MQ=255)                                                                                                            cAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAAGa                 <  2:141543/140‑1 (MQ=255)                                                                                                              gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGTCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAAGATa               <  1:360338/140‑1 (MQ=255)                                                                                                                           tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAAGATAATGTGCTGACGtt  >  2:98617/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    CCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAAGATAATGTGCTGACGTT  >  NC_000913/2210355‑2210616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑