Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,217,643	T→C	L305L (TTA→TTG)	yehY ←	glycine betaine ABC transporter membrane subunit YehY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,217,643	0	T	C	100.0%	78.7 / NA	22	L305L (TTA→TTG)	yehY	glycine betaine ABC transporter membrane subunit YehY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

TGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCA  >  NC_000913/2217513‑2217770                                                                                                                                   |                                                                                                                                tgctTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACa                                                                                                                        >  2:267286/1‑140 (MQ=255)                                        cTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTg                                                                                 >  1:349476/1‑140 (MQ=255)                                        cTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTg                                                                                 >  2:329580/1‑140 (MQ=255)                                           cgcCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGgcg                                                                              <  1:335568/140‑1 (MQ=255)                                                        cgcAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACAt                                                                 <  2:349476/140‑1 (MQ=255)                                                           aaTCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATcgc                                                              <  1:267286/140‑1 (MQ=255)                                                                           aCAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCaa                                              >  2:347950/1‑140 (MQ=255)                                                                            cAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCaaa                                             <  1:347950/140‑1 (MQ=255)                                                                                 tGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGAc                                        >  1:105461/1‑140 (MQ=255)                                                                                  gCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGa                                         <  1:354049/138‑1 (MQ=255)                                                                                  gCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGa                                         >  2:354049/1‑138 (MQ=255)                                                                                  gCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACt                                       >  2:164267/1‑140 (MQ=255)                                                                                   cACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTa                                      >  2:223201/1‑140 (MQ=255)                                                                                      cATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCa                                   <  2:105461/140‑1 (MQ=255)                                                                                              cccGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCgcgc                           <  1:223201/140‑1 (MQ=255)                                                                                                        gcgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgca                 <  1:329580/140‑1 (MQ=255)                                                                                                        gcgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgca                 >  2:153340/1‑140 (MQ=255)                                                                                                         cgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcag                <  1:60242/140‑1 (MQ=255)                                                                                                         cgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcag                >  2:60242/1‑140 (MQ=255)                                                                                                           cAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcagca              <  1:164267/140‑1 (MQ=255)                                                                                                             gAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAg            <  1:153340/140‑1 (MQ=255)                                                                                                                        aaCGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCa  <  2:202477/139‑1 (MQ=255)                                                                                                                        aaCGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCa  >  1:202477/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                TGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCA  >  NC_000913/2217513‑2217770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑