Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,298,514	G→A	H69Y (CAT→TAT)	napB ←	periplasmic nitrate reductase cytochrome c550 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,298,514	0	G	A	100.0%	73.8 / NA	22	H69Y (CAT→TAT)	napB	periplasmic nitrate reductase cytochrome c550 protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

GCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGC  >  NC_000913/2298378‑2298640                                                                                                                                         |                                                                                                                               gcgcCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACgg                                                                                                                             >  2:181289/1‑140 (MQ=255)   gcCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGa                                                                                                                           >  1:130908/1‑140 (MQ=255)    cccACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGAt                                                                                                                          >  1:246960/1‑140 (MQ=255)             ccGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTggcgg                                                                                                                 <  2:314157/140‑1 (MQ=255)             ccGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTggcgg                                                                                                                 <  2:130908/140‑1 (MQ=255)             ccGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTggcgg                                                                                                                 <  1:94755/140‑1 (MQ=255)                tCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTg                                                                                                              <  2:265995/140‑1 (MQ=255)                            aaGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGtt                                                                                                  <  1:181289/140‑1 (MQ=255)                               tGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAg                                                                                               <  1:261693/140‑1 (MQ=255)                                gAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGc                                                                                              >  1:64876/1‑140 (MQ=255)                                 aGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCg                                                                                             >  2:388790/1‑140 (MQ=255)                                                   cgcgcCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTg                                                                                >  2:126475/1‑135 (MQ=255)                                                   cgcgcCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTg                                                                                <  1:126475/135‑1 (MQ=255)                                                         aGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCa                                                                     <  2:334663/140‑1 (MQ=255)                                                                      cTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGcccc                                                        <  1:388790/140‑1 (MQ=255)                                                                                cGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGc                                                           <  1:258469/127‑1 (MQ=255)                                                                                cGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGc                                                           >  2:258469/1‑127 (MQ=255)                                                                                        tGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATAc                                      >  2:322685/1‑140 (MQ=255)                                                                                                  gATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGAc                            >  1:137532/1‑140 (MQ=255)                                                                                                                  tACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGc            >  1:234305/1‑140 (MQ=255)                                                                                                                  tACCTGATAACCTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGc            >  1:117664/1‑140 (MQ=255)                                                                                                                            ccTTCAACGCTATACGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAgccgc  >  2:134646/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               GCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGC  >  NC_000913/2298378‑2298640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑