Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,351,149	T→C	D409G (GAC→GGC)	glpT ←	sn‑glycerol 3‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,351,149	0	T	C	100.0%	52.1 / NA	15	D409G (GAC→GGC)	glpT	sn‑glycerol 3‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GCCATTAGCCTCCGTTGCGTTCTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGCCAGTTCCAGCGCATGCAGACCGATCAGCA  >  NC_000913/2351012‑2351278                                                                                                                                          |                                                                                                                                  gCCATTAGCCTCCGTTGCGTTCTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGccc                                                                                                                                 >  1:189389/1‑140 (MQ=255)  ccATTAGCCTCCGTTGGTTTCTTGCAGTAATTGTTCATGGCGCCGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCa                                                                                                                                <  2:304293/140‑1 (MQ=255)  ccATTAGCCTCCGTTGCGTTCTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCa                                                                                                                                <  1:280767/140‑1 (MQ=255)                    ttCTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATcg                                                                                                              >  2:321433/1‑140 (MQ=255)                               aTTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCgccgcc                                                                                                   >  1:188699/1‑140 (MQ=255)                                 tGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCAc                                                                                                 >  2:91733/1‑140 (MQ=255)                                     cATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGaa                                                                                             <  2:188699/140‑1 (MQ=255)                                          cGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGcc                                                                                        <  1:91733/140‑1 (MQ=255)                                           gACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGccc                                                                                       <  2:374240/140‑1 (MQ=255)                                                                      tCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGccc                                                            >  2:194835/1‑140 (MQ=255)                                                                                        gAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCt                                          >  1:105615/1‑140 (MQ=255)                                                                                              tgcCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTc                                    >  1:57305/1‑140 (MQ=255)                                                                                                   ccAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGc                               <  1:183298/140‑1 (MQ=255)                                                                                                                     ccgccATCCCAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGCCAGTTCCAGCGCATGCAg             >  2:382038/1‑140 (MQ=255)                                                                                                                              cAGCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGCCAGTTCCAGCGCATGCAGACCGATCAg    <  1:321433/140‑1 (MQ=255)                                                                                                                                gCCGAAGAAGCCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGCCAGTTCCAGCGCATGCAGACCGATCAGCa  >  2:89079/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  GCCATTAGCCTCCGTTGCGTTCTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTCCACGGTGTAGCCAACAATCGCGCTCGCCGCCACCGAACCGCCCAGGTAACCAAACAGCCCGGTAAAGCCCGCTGCCGTACCTGCCGCTTTTTTCGGTGCCAGTTCCAGCGCATGCAGACCGATCAGCA  >  NC_000913/2351012‑2351278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑