Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,381,437	T→C	Y36C (TAT→TGT)	elaA ←	putative N‑acetyltransferase ElaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,381,437	0	T	C	100.0%	143.8 / NA	39	Y36C (TAT→TGT)	elaA	putative N‑acetyltransferase ElaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

AATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTA  >  NC_000913/2381301‑2381564                                                                                                                                         |                                                                                                                                aaTCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAg                                                                                                                              <  2:389649/140‑1 (MQ=255)             tataACGACCGGCTCACGATCATCATCACTTTTCAGAATCCTCGCATACGTCACCAGTTCATCATTTTTCCAACCGATAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTTTGCt                                                                                                                  <  2:375765/140‑1 (MQ=255)               taACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCg                                                                                                                <  2:170200/140‑1 (MQ=255)                     ccGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCg                                                                                                                >  1:200879/1‑134 (MQ=255)                     ccGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCg                                                                                                                <  2:200879/134‑1 (MQ=255)                     ccGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGacaaca                                                                                                          >  1:282945/1‑140 (MQ=255)                     ccGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGacaaca                                                                                                          >  2:32477/1‑140 (MQ=255)                     ccGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGacaaca                                                                                                          >  1:418473/1‑140 (MQ=255)                         cTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACaacaaaca                                                                                                      >  1:298482/1‑140 (MQ=255)                                  atcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAAc                                                                                             <  2:418473/140‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAAc                                                                                             >  1:407114/1‑139 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAAc                                                                                             <  2:407114/139‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            <  2:298482/140‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            <  1:43625/140‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            >  2:219650/1‑140 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            <  1:32477/140‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            <  1:219650/140‑1 (MQ=255)                                   tcatcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACg                                                                                            >  2:370336/1‑140 (MQ=255)                                    catcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGc                                                                                           <  1:370336/140‑1 (MQ=255)                                      tcaCTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAg                                                                                         <  2:44899/140‑1 (MQ=255)                                             tCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGtaat                                                                                  <  2:245278/140‑1 (MQ=255)                                                   tCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCa                                                                            <  2:282945/140‑1 (MQ=255)                                                                      ttCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATa                                                         >  2:60621/1‑140 (MQ=255)                                                                        catcatTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAAt                                                       >  2:272604/1‑140 (MQ=255)                                                                           catTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCa                                                    <  2:55725/140‑1 (MQ=255)                                                                              ttttCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCagag                                                 <  1:60621/140‑1 (MQ=255)                                                                                ttCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTg                                               >  2:96565/1‑140 (MQ=255)                                                                                     aCCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCa                                          >  1:142442/1‑140 (MQ=255)                                                                                           aaTATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCtt                                    >  1:102066/1‑140 (MQ=255)                                                                                            atatGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTg                                   <  2:142442/140‑1 (MQ=255)                                                                                                ggCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCAt                               >  2:294704/1‑140 (MQ=255)                                                                                                   gATTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCa                            >  1:11093/1‑140 (MQ=255)                                                                                                    aTTATCCCCCGTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCaa                           >  1:185057/1‑140 (MQ=255)                                                                                                              gTCAAATCATCACCATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGct                 <  1:7412/140‑1 (MQ=255)                                                                                                                          ccATCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGAt     <  2:11093/140‑1 (MQ=255)                                                                                                                            aTCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGt   <  2:102066/140‑1 (MQ=255)                                                                                                                            aTCGATATCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGt   <  1:63803/140‑1 (MQ=255)                                                                                                                                atatCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTa  <  2:371637/137‑1 (MQ=255)                                                                                                                                atatCCTGACAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTa  >  1:371637/1‑137 (MQ=255)                                                                                                                                         |                                                                                                                                AATCACCCGACCTATAACGACCGGCTCAAGATCATCATCACTTTTCAGAATCCTCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTA  >  NC_000913/2381301‑2381564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑