Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,386,814	T→C	L319L (TTA→CTA)	yfbL →	putative peptidase YfbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,814	0	T	C	100.0%	36.7 / NA	11	L319L (TTA→CTA)	yfbL	putative peptidase YfbL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

TTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAA  >  NC_000913/2386704‑2386906                                                                                                               |                                                                                             ttACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTtctc                                                                 <  2:188427/140‑1 (MQ=255)      gACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGc                                                            <  1:322008/140‑1 (MQ=255)         acCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAgg                                                             >  2:366878/1‑136 (MQ=255)         acCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCggg                                                         >  2:227335/1‑140 (MQ=255)         acCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAACGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAgg                                                             <  1:366878/136‑1 (MQ=255)                               aaTACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGcaca                                   <  1:373560/140‑1 (MQ=255)                                               tGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                   <  1:227335/140‑1 (MQ=255)                                                     cGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAGCGACATGCACattttatt                            >  1:94025/1‑125 (MQ=255)                                                     cGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatt                            <  2:94025/125‑1 (MQ=255)                                                              aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGGACATTTTTTTTTTCCTCATCTGTTTTTAGATggg    >  1:212828/1‑138 (MQ=255)                                                                tGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaa  <  2:14871/140‑1 (MQ=255)                                                                                                               |                                                                                             TTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAA  >  NC_000913/2386704‑2386906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑