Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,406,148	T→C	N165S (AAC→AGC)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,406,148	0	T	C	100.0%	77.2 / NA	22	N165S (AAC→AGC)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

ATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAC  >  NC_000913/2406012‑2406272                                                                                                                                         |                                                                                                                             atCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCa                                                                                                                           <  2:198715/140‑1 (MQ=255) atCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTCTTGGCGAGGTACGCAGGCTCa                                                                                                                           <  2:297323/140‑1 (MQ=255)        ttattaAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCtt                                                                                                                    >  1:341822/1‑140 (MQ=255)                cGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGANGTACGCAGGCTCAATGCCTTAAACg                                                                                                               <  2:125079/137‑1 (MQ=255)                cGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACg                                                                                                               >  1:125079/1‑137 (MQ=255)                 gTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGa                                                                                                           <  2:273646/140‑1 (MQ=255)                                      aaCGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCAt                                                                                      >  2:301391/1‑140 (MQ=255)                                          ggCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCCCTGCGCAGTACCAGTGTGGTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCaaa                                                                                  >  2:306080/1‑140 (MQ=255)                                            cTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATc                                                                                <  2:341822/140‑1 (MQ=255)                                             tAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATcc                                                                               <  2:272106/140‑1 (MQ=255)                                                     tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                                       >  1:224220/1‑140 (MQ=255)                                                                       aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGc                                                     >  1:156077/1‑140 (MQ=255)                                                                              cGCCTTTTTGCAATATGTATTCCGCTGCGCGGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAg                                              <  2:309901/140‑1 (MQ=255)                                                                                   ttttGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCAtt                                         <  2:156077/140‑1 (MQ=255)                                                                                                      cTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATcc                      >  1:312091/1‑140 (MQ=255)                                                                                                          gcAGTACCAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAgcg                  <  1:301391/140‑1 (MQ=255)                                                                                                                 cAGTGTGTTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAAttt           <  1:410432/140‑1 (MQ=255)                                                                                                                   gtgtgtTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCg         >  1:206554/1‑140 (MQ=255)                                                                                                                    tgtgtTGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCgg        >  1:47901/1‑140 (MQ=255)                                                                                                                        ttGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAAttt           <  2:294216/133‑1 (MQ=255)                                                                                                                        ttGGCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAAttt           >  1:294216/1‑133 (MQ=255)                                                                                                                          ggCGAGGTACGCAGGCTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAc  >  1:309036/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             ATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAC  >  NC_000913/2406012‑2406272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑