Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,453	A→G	V18A (GTT→GCT)	eutD ←	phosphate acetyltransferase EutD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,453	0	A	G	100.0%	74.8 / NA	21	V18A (GTT→GCT)	eutD	phosphate acetyltransferase EutD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

CGCCACGCCGTGACTGAGCGCAAACTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGATCATC  >  NC_000913/2573323‑2573574                                                                                                                                   |                                                                                                                          cGCCACGCCGTGACTGAGCGCAAACTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATAGTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTgg                                                                                                                  >  2:95722/1‑140 (MQ=255)      cGCCGTGACTGAGCGCAAACTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTggcgggc                                                                                                             >  2:404659/1‑140 (MQ=255)                      aaaCTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCt                                                                                                          >  2:342006/1‑127 (MQ=255)                      aaaCTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCt                                                                                                          <  1:342006/127‑1 (MQ=255)                                           aTTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCa                                                                        >  2:112891/1‑140 (MQ=255)                                                    cAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTc                                                               >  1:202608/1‑140 (MQ=255)                                                            gCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTc                                                       <  1:123248/140‑1 (MQ=255)                                                             cGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCg                                                      <  1:165597/140‑1 (MQ=255)                                                                 gCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGtt                                                                 >  1:308169/1‑125 (MQ=255)                                                                 gCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGtt                                                                 <  2:308169/125‑1 (MQ=255)                                                                 gCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCgttgt                                                  >  1:84726/1‑140 (MQ=255)                                                                     gACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGa                                              >  2:18603/1‑140 (MQ=255)                                                                          tgttgATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTc                                                       >  1:383150/1‑126 (MQ=255)                                                                          tgttgATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTc                                                       <  2:383150/126‑1 (MQ=255)                                                                              gATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCa                                     <  1:142408/140‑1 (MQ=255)                                                                                                 tttCAGCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGt                  <  1:18603/140‑1 (MQ=255)                                                                                                      gCACACGTTGGTCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGc             <  1:330555/140‑1 (MQ=255)                                                                                                                 tCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGatcatc  >  2:140392/1‑140 (MQ=255)                                                                                                                 tCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGatcatc  <  1:140392/140‑1 (MQ=255)                                                                                                                 tCTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTCGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGatcatc  <  1:112891/140‑1 (MQ=255)                                                                                                                  cTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGatcatc  >  1:238024/1‑139 (MQ=255)                                                                                                                  cTAACGCATCCGGAAAAGCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGatcatc  <  2:238024/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                          CGCCACGCCGTGACTGAGCGCAAACTGACGAAGTTCAAACGGATTGGCGACCAGAATGGGCGTTGCCAGACCTTGTTGATGTAAATATTGCGCGGCTTTCAGCACACGTTGGTCTAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTCGTTACGCTCAGGATCATC  >  NC_000913/2573323‑2573574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑