Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	294,481	G→A	R107* (CGA→TGA)	yagM ←	CP4‑6 prophage; protein YagM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	294,481	0	G	A	100.0%	103.9 / NA	30	R107* (CGA→TGA)	yagM	CP4‑6 prophage; protein YagM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

TTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAA  >  NC_000913/294344‑294611                                                                                                                                          |                                                                                                                                   tttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGc                                                                                                                                  >  2:14697/1‑140 (MQ=255)  ttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTTCTCTTCCAGAGTTACTTCTGTTTTTCCTCAGCt                                                                                                                                 <  2:403251/140‑1 (MQ=255)       aTCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGAt                                                                                                                            <  1:14697/140‑1 (MQ=255)                  tGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGctttct                                                                                                                 >  1:34534/1‑140 (MQ=255)                         atcatTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATag                                                                                                          <  1:10055/140‑1 (MQ=255)                         atcatTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATag                                                                                                          <  1:257261/140‑1 (MQ=255)                         atcatTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATag                                                                                                          >  2:10055/1‑140 (MQ=255)                            atTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAgaga                                                                                                       <  2:182716/140‑1 (MQ=255)                                 cGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATag                                                                                                          >  2:56765/1‑132 (MQ=255)                                 cGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATag                                                                                                          <  1:56765/132‑1 (MQ=255)                                   ttCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTaa                                                                                                >  1:96301/1‑140 (MQ=255)                                                           gggATTTTTAATGGACTCATGATGTTCGTTTTCCAGAGTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTGTTATAGAGAACATTAACAGGTCATTTTTTATTTCATTTAt                                                                        >  2:370746/1‑140 (MQ=255)                                                               tttttAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGAc                                                                    <  1:126413/140‑1 (MQ=255)                                                               tttttAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGAc                                                                    <  1:30343/140‑1 (MQ=255)                                                               tttttAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGAc                                                                    <  1:132067/140‑1 (MQ=255)                                                                 tttAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCa                                                                  <  2:367991/140‑1 (MQ=255)                                                                                atgTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCa                                                   <  2:285805/140‑1 (MQ=255)                                                                                 tgTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCAt                                                  <  2:389982/140‑1 (MQ=255)                                                                                 tgTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCAt                                                  <  1:258662/140‑1 (MQ=255)                                                                                           ccAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAata                                        <  2:416596/140‑1 (MQ=255)                                                                                           ccAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAata                                        <  2:96301/140‑1 (MQ=255)                                                                                                           tACTCTTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATc                        >  1:250454/1‑140 (MQ=255)                                                                                                             ctctTCCAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCca                      <  2:250454/140‑1 (MQ=255)                                                                                                                  ccAGAGTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACt                 >  2:25708/1‑140 (MQ=255)                                                                                                                     gagTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTcc              >  2:220500/1‑140 (MQ=255)                                                                                                                     gagTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTcc              >  1:42167/1‑140 (MQ=255)                                                                                                                       gTTACTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGc            <  1:334414/140‑1 (MQ=255)                                                                                                                          aCTTCTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTAt         >  2:1306/1‑140 (MQ=255)                                                                                                                              cTGTTTTACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACg     <  2:379432/140‑1 (MQ=255)                                                                                                                                 ttttACCTCAGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGaa  <  1:286746/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCTTTTATAGAGAACTTTAACAGGTCATTTTTTATTTCATTTATTGACCAGTTATGACGATTGCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAA  >  NC_000913/294344‑294611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑