Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,721,901	T→C	V650A (GTT→GCT)	patZ →	peptidyl‑lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,901	0	T	C	100.0%	47.8 / NA	14	V650A (GTT→GCT)	patZ	peptidyl‑lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

GGTGTGGAGTGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCAC  >  NC_000913/2721765‑2722024                                                                                                                                         |                                                                                                                            ggTGTGGAGTGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGAtgctgc                                                                                                                          <  1:196450/140‑1 (MQ=255)    gtgGAGTGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAgg                                                                                                                       >  2:402312/1‑140 (MQ=255)    gtgGAGTGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAgg                                                                                                                       >  2:408464/1‑140 (MQ=255)          tGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAg                                                                                                                 <  1:402312/140‑1 (MQ=255)          tGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAg                                                                                                                 <  1:408464/140‑1 (MQ=255)                                       aCTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGa                                                                                    <  2:159256/140‑1 (MQ=255)                                                              tGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAg                                                             >  1:62169/1‑140 (MQ=255)                                                                gCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTAAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCg                                                           >  2:207101/1‑140 (MQ=255)                                                                    gCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTAAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTg                                                       <  1:207101/140‑1 (MQ=255)                                                                     cTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTgg                                                      >  1:93540/1‑140 (MQ=255)                                                                                   aGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTg                                        <  2:62169/140‑1 (MQ=255)                                                                                               gTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGc                            >  1:276815/1‑140 (MQ=255)                                                                                                         tCGTGCGCGCAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAATTTa                  >  1:132326/1‑140 (MQ=255)                                                                                                                cgcAGTGCGCTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCt           <  2:157912/140‑1 (MQ=255)                                                                                                                         cTACGCCCATTGGATGCTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCAc  <  2:93540/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                            GGTGTGGAGTGGCGTCCTGAAGATCAAGCCGTCGTCGCACTGCCGCCGCTGAACATGAACCTGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCAC  >  NC_000913/2721765‑2722024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑