Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,976	A→G	H417R (CAC→CGC)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,976	0	A	G	100.0%	116.5 / NA	32	H417R (CAC→CGC)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/18);  total (14/18)

GTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTT  >  NC_000913/2723841‑2724111                                                                                                                                        |                                                                                                                                        gTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTAt                                                                                                                                     >  2:279818/1‑140 (MQ=255)  taataaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATc                                                                                                                                    <  2:115228/140‑1 (MQ=255)       aCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                               <  2:39813/140‑1 (MQ=255)         cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                               >  1:167476/1‑138 (MQ=255)         cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                               <  2:167476/138‑1 (MQ=255)          tGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTg                                                                                                                            <  1:315515/140‑1 (MQ=255)            aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                          >  1:400568/1‑140 (MQ=255)                 gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                               <  2:95993/130‑1 (MQ=255)                 gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                               >  1:95993/1‑130 (MQ=255)                  cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcg                                                                                                                                   >  1:142749/1‑125 (MQ=255)                  cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcg                                                                                                                                   <  2:142749/125‑1 (MQ=255)                                    ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                                                                                  <  2:148195/140‑1 (MQ=255)                                                                cGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      <  1:125586/140‑1 (MQ=255)                                                                cGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      <  1:146464/140‑1 (MQ=255)                                                                        gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      >  1:213806/1‑132 (MQ=255)                                                                        gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      <  2:213806/132‑1 (MQ=255)                                                                             ggCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCg                                                         >  2:202868/1‑140 (MQ=255)                                                                               cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      <  1:329757/125‑1 (MQ=255)                                                                               cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      >  2:329757/1‑125 (MQ=255)                                                                               cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc                                                       >  2:202705/1‑140 (MQ=255)                                                                                gcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAcc                                                      <  1:330837/140‑1 (MQ=255)                                                                                   acaGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGat                                                   >  2:84503/1‑140 (MQ=255)                                                                                       cGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat                                               <  1:202868/140‑1 (MQ=255)                                                                                                   cTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat                                               >  2:346531/1‑128 (MQ=255)                                                                                                   cTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat                                               <  1:346531/128‑1 (MQ=255)                                                                                                     ggAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGt                                 <  2:328723/140‑1 (MQ=255)                                                                                                              ccGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGGTTATCCGGTGAAGTTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAc                        >  2:419679/1‑140 (MQ=255)                                                                                                              ccGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAc                        <  2:400568/140‑1 (MQ=255)                                                                                                                  gAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCg                    <  1:122178/140‑1 (MQ=255)                                                                                                                        accaccATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGt              <  1:202705/140‑1 (MQ=255)                                                                                                                                  ttAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttg    >  2:77073/1‑140 (MQ=255)                                                                                                                                    aaGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCACCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgtt  >  2:374931/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTT  >  NC_000913/2723841‑2724111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑