Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,773,381	A→G	I22V (ATC→GTC)	yfjW →	CP4‑57 prophage; uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,773,381	0	A	G	100.0%	114.0 / NA	32	I22V (ATC→GTC)	yfjW	CP4‑57 prophage; uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/18);  total (14/18)

GGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAA  >  NC_000913/2773245‑2773509                                                                                                                                         |                                                                                                                                 ggTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCt                                                                                                                               >  2:377310/1‑140 (MQ=255)    tGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTgg                                                                                                                            <  1:342465/140‑1 (MQ=255)      cTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCa                                                                                                                          <  2:341764/140‑1 (MQ=255)         gAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCAttt                                                                                                                       <  2:161330/140‑1 (MQ=255)            aGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTc                                                                                                                    <  2:122344/140‑1 (MQ=255)              tCGATGATTTGTTGCGTATGATTAAAAATCAACCGTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATAGACTTTACGGTCTTGGCATTGTTCta                                                                                                                  >  2:371396/1‑140 (MQ=255)                        gttgCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCctct                                                                                                        >  1:408078/1‑140 (MQ=255)                           gCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTAtt                                                                                                     >  1:396625/1‑140 (MQ=255)                               aTGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTAtttttt                                                                                                 <  1:214353/140‑1 (MQ=255)                                 gATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTAtttttttt                                                                                               <  2:111805/140‑1 (MQ=255)                                         aTCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTg                                                                                       <  2:82408/140‑1 (MQ=255)                                            aaCCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAg                                                                                    >  1:244033/1‑140 (MQ=255)                                              ccTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTg                                                                                  >  1:233816/1‑140 (MQ=255)                                                   aaGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGtt                                                                             <  2:97177/140‑1 (MQ=255)                                                        tttATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCtt                                                                        >  2:160262/1‑140 (MQ=255)                                                         ttATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCttt                                                                       <  1:160262/140‑1 (MQ=255)                                                           ataCATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCa                                                                     <  2:244033/140‑1 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCa                                                                     <  2:200003/129‑1 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCa                                                                     >  1:200003/1‑129 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCg                                                          <  1:40671/140‑1 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCg                                                          >  2:173826/1‑140 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCg                                                          >  2:267308/1‑140 (MQ=255)                                                                      tGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCg                                                          <  1:173826/140‑1 (MQ=255)                                                                        cTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCt                                                        <  1:332156/140‑1 (MQ=255)                                                                                tttGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTcac                                                >  2:309364/1‑140 (MQ=255)                                                                                  tGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTGTCAATATTTTACCGCTGCCTTCACaa                                              >  1:293902/1‑140 (MQ=255)                                                                                        aGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTa                                        <  1:377310/140‑1 (MQ=255)                                                                                        aGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTa                                        >  2:293263/1‑140 (MQ=255)                                                                                             gagaAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGt                                   <  1:140115/140‑1 (MQ=255)                                                                                                    tGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATAATTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAAt                            >  1:331291/1‑140 (MQ=255)                                                                                                                    aGCAAAAAAATTGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGa            <  1:9976/140‑1 (MQ=255)                                                                                                                              ttGACTTTACGGTCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATaaaa  <  1:327451/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAA  >  NC_000913/2773245‑2773509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑