Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,882,486	T→C	N51S (AAC→AGC)	casB ←	type I‑E CRISPR system Cascade subunit CasB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,882,486	0	T	C	100.0%	105.4 / NA	30	N51S (AAC→AGC)	casB	type I‑E CRISPR system Cascade subunit CasB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

CCACTATTGGCTAAAGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGTTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAA  >  NC_000913/2882354‑2882610                                                                                                                                     |                                                                                                                             ccACTATTGGCTAAAGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCcca                                                                                                                       >  2:13120/1‑140 (MQ=255)     tATTGGCTAAAGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATCTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCccaacca                                                                                                                   <  1:179530/140‑1 (MQ=255)          gCTAAAGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAgg                                                                                                              >  2:416941/1‑140 (MQ=255)               aGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCa                                                                                                         >  2:408092/1‑140 (MQ=255)               aGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCa                                                                                                         <  1:408092/140‑1 (MQ=255)               aGCTCTTCCCAACGAGATACCTGGTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCa                                                                                                         >  2:197987/1‑140 (MQ=255)                gCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCAc                                                                                                        <  2:50739/140‑1 (MQ=255)                 ctctTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCAcc                                                                                                       <  1:405835/140‑1 (MQ=255)                    ttCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGc                                                                                                    >  1:199864/1‑140 (MQ=255)                    ttCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGc                                                                                                    >  2:172868/1‑140 (MQ=255)                         aaCGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCtata                                                                                               <  1:416941/140‑1 (MQ=255)                          aCGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATaa                                                                                              <  1:344162/140‑1 (MQ=255)                                tACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCa                                                                                        >  1:344707/1‑140 (MQ=255)                                tACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCa                                                                                        >  2:30640/1‑140 (MQ=255)                                    tgttgtTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGa                                                                                    <  2:199864/140‑1 (MQ=255)                                      ttgttTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGata                                                                                  >  1:351200/1‑140 (MQ=255)                                           tGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATcgcg                                                                             >  1:121407/1‑140 (MQ=255)                                                cGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAAtt                                                                        >  2:26728/1‑140 (MQ=255)                                                   ttttttGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATtcat                                                                     >  1:130810/1‑140 (MQ=255)                                                   ttttttGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATtcat                                                                     <  1:29658/140‑1 (MQ=255)                                                               aTGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGaaa                                                         <  2:412872/140‑1 (MQ=255)                                                                               tttCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTgcgc                                         <  2:130810/140‑1 (MQ=255)                                                                                   cTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACAt                                     <  2:344707/140‑1 (MQ=255)                                                                                                       aTGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGttgttg                 >  1:143496/1‑140 (MQ=255)                                                                                                       aTGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGttgttg                 >  1:102839/1‑140 (MQ=255)                                                                                                        tGCGCAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGc                <  2:351200/140‑1 (MQ=255)                                                                                                           gcAAAAGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAg             <  2:143496/140‑1 (MQ=255)                                                                                                             aaaaGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGc           >  1:394602/1‑140 (MQ=255)                                                                                                              aaaGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCt          <  1:172868/140‑1 (MQ=255)                                                                                                              aaaGAGCCTGCTGGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCt          >  1:330597/1‑140 (MQ=255)                                                                                                                      tgctgGTGACGTGGGCTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATaa  <  2:119462/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                             CCACTATTGGCTAAAGCTCTTCCCAACGAGATACCTGTTGTTTGCTCCGATTTTTTGTCCTGATGTCGGATGACATTCTTTCCTGCGCTCAGGCAAAACACCATGCGCAAAAGAGCCTGCTGGTGACGTGGGTTTTCCCAACCAAAAGGTTGCACCAGCCTATAAAACGCAGGGATATCGCGTAATTCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAA  >  NC_000913/2882354‑2882610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑