Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,986	A→G	V254A (GTT→GCT)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,986	0	A	G	100.0%	85.5 / NA	24	V254A (GTT→GCT)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

ATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCC  >  NC_000913/2919850‑2920113                                                                                                                                         |                                                                                                                                aTGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGcat                                                                                                                              >  2:116187/1‑140 (MQ=255)     cccATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCAtt                                                                                                                          >  2:269165/1‑140 (MQ=255)             gcgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCtt                                                                                                                  <  1:129212/140‑1 (MQ=255)               gcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTg                                                                                                                >  2:233694/1‑140 (MQ=255)                                aTGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTtc                                                                                               >  2:248012/1‑140 (MQ=255)                                      gTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAag                                                                                         <  1:233694/140‑1 (MQ=255)                                        cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGc                                                                                                  >  2:346404/1‑129 (MQ=255)                                        cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGc                                                                                                  <  1:346404/129‑1 (MQ=255)                                        cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagca                                                                                       <  2:46038/140‑1 (MQ=255)                                         gcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                                                                                      <  1:269165/140‑1 (MQ=255)                                          cgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcagc                                                                                     >  1:122207/1‑140 (MQ=255)                                              gggCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATg                                                                                 <  2:35919/140‑1 (MQ=255)                                                         tCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                                                                                      <  2:171701/124‑1 (MQ=255)                                                         tCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                                                                                      >  1:171701/1‑124 (MQ=255)                                                          cGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                                                                                      >  1:411237/1‑123 (MQ=255)                                                          cGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                                                                                      <  2:411237/123‑1 (MQ=255)                                                          cGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGAt                                                                     <  2:122207/140‑1 (MQ=255)                                                          cGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGAt                                                                     <  1:248012/140‑1 (MQ=255)                                                                       tCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATAcg                                                        >  2:199974/1‑140 (MQ=255)                                                                                                            cgccgcATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCaa                                <  2:247527/127‑1 (MQ=255)                                                                                                            cgccgcATGGATCTTCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCaa                                >  1:247527/1‑127 (MQ=255)                                                                                                                         ttCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCt      <  1:86338/140‑1 (MQ=255)                                                                                                                         ttCGGCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCt      >  2:378/1‑140 (MQ=255)                                                                                                                             gCATAGGTAAGAGCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGcc  <  1:116187/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                ATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCC  >  NC_000913/2919850‑2920113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑