Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,706	(A)7→6	coding (370/465 nt)	yhaV →	ribosome‑dependent mRNA interferase toxin YhaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,700	0	A	.	100.0%	256.1 / NA	60	coding (364/465 nt)	yhaV	ribosome‑dependent mRNA interferase toxin YhaV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (39/21);  total (39/21)

TGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCA  >  NC_000913/3277567‑3277828                                                                                                                                      |                                                                                                                                 tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                                                            >  2:297455/1‑139 (MQ=255) tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                                                            >  1:950088/1‑139 (MQ=255) tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTa                                                                                                                                  <  1:756745/134‑1 (MQ=255) tGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTa                                                                                                                                  >  2:756745/1‑134 (MQ=255)  gggAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           <  2:985048/140‑3 (MQ=255)  gggAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           <  1:819743/140‑3 (MQ=255)  gggAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           <  1:403284/140‑3 (MQ=255)  gggAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           <  1:297455/140‑3 (MQ=255)    gAAAAATAAAGACTCGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacaga                                                                                                                         <  2:1059735/140‑5 (MQ=255)       aaaTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGc                                                                                                                     <  1:480140/140‑1 (MQ=255)         aTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCt                                                                                                                   <  1:774018/140‑1 (MQ=255)         aTAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCt                                                                                                                   <  2:950088/140‑1 (MQ=255)                  ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           >  2:1179262/1‑122 (MQ=255)                  ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                                                           <  1:1179262/124‑3 (MQ=255)                  ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTAt                                                                                                          >  1:1023405/1‑140 (MQ=255)                  ggTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTAt                                                                                                          >  2:317615/1‑140 (MQ=255)                     cACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTa                                                                                                           >  2:79308/1‑136 (MQ=255)                     cACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTa                                                                                                           <  1:79308/136‑1 (MQ=255)                               aTTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGtt                                                                                             <  1:323170/140‑1 (MQ=255)                                    gTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa                                                                                        >  1:843314/1‑140 (MQ=255)                                    gTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa                                                                                        >  1:667369/1‑140 (MQ=255)                                          gTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGAc                                                                                  >  2:472194/1‑140 (MQ=255)                                                   gTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                         <  2:103241/140‑1 (MQ=255)                                                    tctcTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGc                                                                        >  2:986439/1‑140 (MQ=255)                                                                 tataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                                                           <  2:1023405/140‑1 (MQ=255)                                                                  ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                          >  2:23810/1‑140 (MQ=255)                                                                  ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                          >  2:103606/1‑140 (MQ=255)                                                                    aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                        >  1:400716/1‑140 (MQ=255)                                                                     gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                        <  1:590350/139‑1 (MQ=255)                                                                     gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                        >  2:590350/1‑139 (MQ=255)                                                                        aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                                                    >  2:580594/1‑140 (MQ=255)                                                                         aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                   <  2:728866/140‑1 (MQ=255)                                                                             gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                                                      <  2:791445/133‑1 (MQ=255)                                                                             gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                                                      >  1:791445/1‑133 (MQ=255)                                                                                       tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                     >  1:98736/1‑140 (MQ=255)                                                                                       tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                     >  2:104959/1‑140 (MQ=255)                                                                                         atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAcc                                   <  2:244442/140‑1 (MQ=255)                                                                                            cTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt                                >  2:387781/1‑140 (MQ=255)                                                                                             tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt                               >  2:692281/1‑140 (MQ=255)                                                                                             tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt                               >  1:85453/1‑140 (MQ=255)                                                                                              gggATGGATGAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTg                              >  1:616932/1‑140 (MQ=255)                                                                                                gatggatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                            <  1:580594/140‑1 (MQ=255)                                                                                                 atggatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                           <  2:64849/140‑1 (MQ=255)                                                                                                 atggatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                           <  1:538347/140‑1 (MQ=255)                                                                                                 atggatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                           <  2:192942/140‑1 (MQ=255)                                                                                                    gatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                        >  2:1058337/1‑140 (MQ=255)                                                                                                    gatgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                        >  2:860628/1‑140 (MQ=255)                                                                                                     atgAACGATGAAAACTCTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                          <  2:1045844/137‑1 (MQ=255)                                                                                                     atgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                          >  1:1045844/1‑137 (MQ=255)                                                                                                     atgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtgt                       <  2:400716/140‑1 (MQ=255)                                                                                                     atgAACGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtgt                       >  1:59805/1‑140 (MQ=255)                                                                                                         aCGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                           <  2:719184/132‑1 (MQ=255)                                                                                                         aCGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                           >  1:719184/1‑132 (MQ=255)                                                                                                         aCGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac                   >  2:1202031/1‑140 (MQ=255)                                                                                                         aCGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac                   >  2:709211/1‑140 (MQ=255)                                                                                                         aCGATGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac                   >  2:709666/1‑140 (MQ=255)                                                                                                            aTGAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                >  1:1135773/1‑140 (MQ=255)                                                                                                              gAAAACACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGc              >  1:156223/1‑140 (MQ=255)                                                                                                               aaaaCACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCTACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc             >  2:1041040/1‑140 (MQ=255)                                                                                                               aaaaCACTCTGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc             >  1:957006/1‑140 (MQ=255)                                                                                                                      tctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa      <  2:39569/140‑1 (MQ=255)                                                                                                                      tctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa      <  2:85453/140‑1 (MQ=255)                                                                                                                      tctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa      <  1:692281/140‑1 (MQ=255)                                                                                                                       ctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc     >  1:843422/1‑140 (MQ=255)                                                                                                                       ctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc     >  1:617583/1‑140 (MQ=255)                                                                                                                       ctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc     >  1:908078/1‑140 (MQ=255)                                                                                                                       ctGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc     >  2:407607/1‑140 (MQ=255)                                                                                                                        tGCGCACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACg    >  2:887289/1‑140 (MQ=255)                                                                                                                         gcgcACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGc   >  2:1001782/1‑140 (MQ=255)                                                                                                                         gcgcACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGc   >  2:363506/1‑140 (MQ=255)                                                                                                                          cgcACCTACGGT‑AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCa  >  1:865553/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 TGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCA  >  NC_000913/3277567‑3277828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑