Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,713	(T)7→8	intergenic (+30/+44)	metV → / ← amiC	tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,706	1	.	T	100.0%	112.8 / NA	33	intergenic (+23/+51)	metV/amiC	tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (19/14);  total (19/14)

ATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCCTGCT  >  NC_000913/2947574‑2947836                                                                                                                                      |                                                                                                                                    aTCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑tttt‑ttt                                                                                                                             >  1:1134231/1‑140 (MQ=255)            tgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGtc                                                                                                                   <  1:1114219/140‑1 (MQ=255)                         gTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATaaaa                                                                                                         >  2:181033/1‑137 (MQ=255)                         gTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATaaaa                                                                                                         <  1:181033/137‑1 (MQ=255)                          tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTTACATCCTAAAGGGGGATTTC‑TTTGATTCTGGGCGGCCATAAAAAAgc                                                                                                     >  2:546378/1‑140 (MQ=14)                          tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAgc                                                                                                     >  2:722654/1‑140 (MQ=255)                          tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAgc                                                                                                     >  1:333574/1‑140 (MQ=255)                           aCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAgcg                                                                                                    >  1:398818/1‑140 (MQ=255)                                   gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAg                                                                                            <  2:856592/140‑1 (MQ=255)                                    cgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgc                                                                                           <  1:546378/140‑1 (MQ=255)                                             gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTa                                                                                  <  2:1134231/140‑1 (MQ=255)                                             cgagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTa                                                                                  <  1:1122220/138‑1 (MQ=255)                                                 ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCTCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTatcat                                                                              >  1:306621/1‑140 (MQ=255)                                                 ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTatcat                                                                              >  1:266970/1‑140 (MQ=255)                                                 ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTatcat                                                                              >  2:1211919/1‑140 (MQ=255)                                                           cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGTACACCCTAACGGGGG‑TTTTCTTTGATTCTGGGCGCCCCAAAAAAAGCGCCATTCGGCGCCTTTTTTCCATCCCCTtcctg                                                                    >  2:1122220/1‑137 (MQ=21)                                                           cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCg                                                                    >  1:398682/1‑140 (MQ=255)                                                           cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCg                                                                    >  1:1204929/1‑140 (MQ=255)                                                            gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGc                                                                   <  1:1211919/140‑1 (MQ=255)                                                              cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCa                                                                 >  1:108382/1‑140 (MQ=255)                                                                           cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATc                                                    >  2:1061669/1‑140 (MQ=255)                                                                           cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATc                                                    >  2:90970/1‑140 (MQ=255)                                                                             aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCgg                                                  >  1:896865/1‑140 (MQ=255)                                                                               ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCa                                                >  2:54154/1‑140 (MQ=255)                                                                                                cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATccc                               <  2:1198981/140‑1 (MQ=255)                                                                                                  gCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg                             <  2:647527/140‑1 (MQ=255)                                                                                                  gCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg                             <  2:108382/140‑1 (MQ=255)                                                                                                  gCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg                             <  2:266970/140‑1 (MQ=255)                                                                                                  gCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg                             <  1:1080626/140‑1 (MQ=255)                                                                                                   cccccGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGc                            <  2:1213922/140‑1 (MQ=255)                                                                                                   cccccGCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGc                            <  2:72782/140‑1 (MQ=255)                                                                                                        gCAACCAATTATTGAACACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGaa                       >  1:1091417/1‑140 (MQ=255)                                                                                                                      aaCACCCTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACt         >  2:84589/1‑140 (MQ=255)                                                                                                                           ccTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTACTCCCCGCAAGAATAGACTCCGCAACTTCctg    >  1:256037/1‑140 (MQ=255)                                                                                                                           ccTAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCctg    >  2:378496/1‑140 (MQ=255)                                                                                                                             tAACGGGTGTTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCctgct  >  2:918347/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    ATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCCTGCT  >  NC_000913/2947574‑2947836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑