Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,101,039	(C)7→8	coding (585/720 nt)	yggN ←	putative EcfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,032	1	.	C	96.2%	178.9 / ‑0.1	52	coding (592/720 nt)	yggN	putative EcfF
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (25/25);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGC  >  NC_000913/3100894‑3101166                                                                                                                                             |                                                                                                                                        aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c                                                                                                                                       <  2:586997/140‑1 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc                                                                                                                                  >  2:759324/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc                                                                                                                                  >  1:1087422/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc                                                                                                                                  >  2:293118/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc                                                                                                                                  >  1:226476/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc                                                                                                                                  >  1:387231/1‑140 (MQ=255)       agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccc                                                                                                                                 >  1:801200/1‑140 (MQ=255)        ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccc                                                                                                                                >  2:1150492/1‑139 (MQ=255)         gATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca                                                                                                                               <  1:977879/140‑3 (MQ=255)           ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg                                                                                                                             <  2:801200/140‑5 (MQ=255)           ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg                                                                                                                             <  1:293118/140‑5 (MQ=255)             attTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCt                                                                                                                            <  2:794603/140‑1 (MQ=255)               tttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTc                                                                                                                          <  1:352669/140‑1 (MQ=255)                  aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa                                                                                                                       <  1:1005835/140‑1 (MQ=255)                  aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa                                                                                                                       >  2:1005835/1‑140 (MQ=255)                   aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAg                                                                                                                      >  2:357667/1‑140 (MQ=255)                      tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcac                                                                                                                   <  2:790593/140‑1 (MQ=255)                       cccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcaca                                                                                                                  <  1:463434/140‑1 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 >  1:504383/1‑140 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  1:576013/140‑1 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 >  2:1049637/1‑140 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  2:504383/140‑1 (MQ=255)                         cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  1:767041/140‑1 (MQ=255)                         cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAtt                                                                                                                <  2:343552/140‑1 (MQ=255)                             cAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGt                                                                                                            >  1:131440/1‑140 (MQ=255)                               gggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATTTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                          <  1:357667/140‑1 (MQ=255)                               gggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  2:1220820/133‑1 (MQ=255)                               gggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 >  1:1220820/1‑133 (MQ=255)                                ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAt                                                                                                         >  1:319476/1‑140 (MQ=255)                                ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAt                                                                                                         >  2:220285/1‑140 (MQ=255)                                    ttGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                                     >  2:1156060/1‑140 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                                     >  2:76184/1‑140 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAtt                                                                                                                >  1:613500/1‑128 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAtt                                                                                                                <  2:613500/128‑1 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  1:222937/1‑140 (MQ=255)                                       cGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAc                                                                                                  <  1:759324/140‑1 (MQ=255)                                                         acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                >  2:776174/1‑140 (MQ=255)                                                           aaCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCt                                                                              >  2:580498/1‑140 (MQ=255)                                                              gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                                                                           <  2:319476/140‑1 (MQ=255)                                                                ggCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  2:226476/140‑1 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTTGAAATCTTTTTCCAGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTATTGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  2:1084122/140‑1 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  1:580498/140‑1 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  1:220285/140‑1 (MQ=255)                                                                    aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc                                                                      <  1:76184/140‑1 (MQ=255)                                                                            cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                                                                   >  2:120594/1‑134 (MQ=255)                                                                            cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                                                                   <  1:120594/134‑1 (MQ=255)                                                                                gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc                                                                      >  1:888631/1‑127 (MQ=255)                                                                                gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc                                                                      <  2:888631/127‑1 (MQ=255)                                                                                      ctgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTcc                                                   >  2:980719/1‑140 (MQ=255)                                                                                                          gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc                               <  2:1014126/140‑1 (MQ=255)                                                                                                           cTTTTTCCCCCCGGTTTGGATGGAAGATTGCACC‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc                              <  2:222937/140‑1 (MQ=255)                                                                                                           cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc                              >  2:612212/1‑140 (MQ=255)                                                                                                                tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCAtt                                 >  2:562626/1‑132 (MQ=255)                                                                                                                tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCAtt                                 <  1:562626/132‑1 (MQ=255)                                                                                                                     tCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTa                    >  1:1205812/1‑140 (MQ=255)                                                                                                                     tCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTa                    >  1:1041062/1‑140 (MQ=255)                                                                                                                                 gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt        >  2:66687/1‑140 (MQ=255)                                                                                                                                 gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt        <  1:1150492/140‑1 (MQ=255)                                                                                                                                  aGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc       >  2:887140/1‑140 (MQ=255)                                                                                                                                   ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCg      >  1:1009095/1‑140 (MQ=255)                                                                                                                                      ttGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg   <  2:1009095/140‑1 (MQ=255)                                                                                                                                      ttGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg   <  1:612212/140‑1 (MQ=255)                                                                                                                                       tGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcgc  >  1:939028/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                        AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGC  >  NC_000913/3100894‑3101166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑