| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 453,367 | (A)5→6 | coding (179/1476 nt) | ampG ← | muropeptide:H(+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 453,362 | 1 | . | A | 96.4% | 193.2 / ‑0.6 | 56 | coding (184/1476 nt) | ampG | muropeptide:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (22/32); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑AAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATC > NC_000913/453223‑453492 | cGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAAttt > 1:100555/1‑140 (MQ=255) gAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑aa < 1:407636/140‑1 (MQ=255) aGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑aaa > 2:928399/1‑140 (MQ=255) ccTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAg < 2:1010539/140‑1 (MQ=255) ccTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑AAAAACGTAAGc > 2:88783/1‑140 (MQ=255) cTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGc > 1:1218289/1‑140 (MQ=255) cTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGc > 1:848744/1‑140 (MQ=255) tGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGcc < 2:221160/140‑1 (MQ=255) tGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGcc < 2:1218289/140‑1 (MQ=255) tGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGcc < 2:707734/140‑1 (MQ=255) gTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTg < 2:732816/140‑1 (MQ=255) gTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTg < 2:492982/140‑1 (MQ=255) gTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTg < 1:828433/140‑1 (MQ=255) gTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTg < 1:195089/140‑1 (MQ=255) tCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGc > 1:1111148/1‑140 (MQ=255) agaAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTa < 2:1189218/140‑1 (MQ=255) aCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCaga > 1:1065490/1‑140 (MQ=255) aCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCaga < 2:342417/140‑1 (MQ=255) aTCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAg > 2:342086/1‑122 (MQ=255) aTCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAg < 1:342086/122‑1 (MQ=255) aTCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAgagaga > 1:416435/1‑140 (MQ=255) tCGCCGCAATGGCAACTAATAACAGGATTCGCGTGGCGAGCAGCCAACGGCGCCGCCGCGCAAAAAATGGAGGCGTGTAGCGGTACATCAGCGGTGTCCGGAGGAATTTAAAAAACGTAGGCGTCGCCTACCAGAGagaa < 2:374248/140‑1 (MQ=255) cgccgcAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGagaag > 1:56290/1‑140 (MQ=255) cgccgcAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGagaag > 1:706296/1‑140 (MQ=255) cgccgcAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGagaag > 2:73436/1‑140 (MQ=255) aaTGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCa < 2:706296/140‑1 (MQ=255) aaTGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑AAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCaa < 1:88783/140‑1 (MQ=255) aCTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCagagag > 2:1206484/1‑124 (MQ=255) aCTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCagagag < 1:1206484/124‑1 (MQ=255) aCTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGttt > 2:666767/1‑140 (MQ=255) aataaCAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTga < 2:1111148/140‑1 (MQ=255) ataaCAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTgag < 1:701277/140‑1 (MQ=255) aaCAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGAt < 1:73436/140‑1 (MQ=255) aCAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTgag < 2:838172/137‑1 (MQ=255) aCAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTgag > 1:838172/1‑137 (MQ=255) gATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCaa > 1:412849/1‑123 (MQ=255) gATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCaa < 2:412849/123‑1 (MQ=255) aTTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGatat > 2:999329/1‑140 (MQ=255) ttGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGAt < 2:1178367/132‑1 (MQ=255) ttGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGAt > 1:1178367/1‑132 (MQ=255) tGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATtct > 1:207191/1‑140 (MQ=255) gCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCg < 2:416435/140‑1 (MQ=255) gCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCg < 2:207191/140‑1 (MQ=255) gCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCg < 1:666767/140‑1 (MQ=255) gCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCg < 2:984947/140‑1 (MQ=255) gCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAAAGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCg < 2:848744/140‑1 (MQ=255) cGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGActg < 2:452349/139‑3 (MQ=255) cGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGActg > 1:452349/1‑137 (MQ=255) cGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGt > 1:560861/1‑140 (MQ=255) agcagcCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCa < 1:269292/140‑1 (MQ=255) ccAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAg < 1:528183/140‑1 (MQ=255) aaCCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGc < 2:56290/140‑1 (MQ=255) gTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCg > 2:827878/1‑140 (MQ=255) gTCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCg > 1:1120576/1‑140 (MQ=255) tCCATCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCgg < 2:560861/140‑1 (MQ=255) tCAGCGGTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGc > 1:1072029/1‑140 (MQ=255) gTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCaa < 2:409449/136‑1 (MQ=255) gTGACCAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCaa > 1:409449/1‑136 (MQ=255) ccAGAGGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAgg < 2:1120576/140‑1 (MQ=255) gagGAATTTAAAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATc < 2:1072029/140‑1 (MQ=255) | CGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTT‑AAAAACGTAAGCCTGGCCTACCAGAGAGAAGAAACCAATGGTTTTGAGATCGATATTCTCGACCGTCATCCAGGCCTGTAAGGTACCGGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATC > NC_000913/453223‑453492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |