Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,053,516	(G)6→7	coding (2663/2745 nt)	torS ←	sensory histidine kinase TorS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,053,510	1	.	G	100.0%	224.1 / NA	61	L890P (CTT→CCT)	torS	sensory histidine kinase TorS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (35/26);  total (35/26)

TTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCC  >  NC_000913/1053378‑1053629                                                                                                                                      |                                                                                                                        ttAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                  <  2:573487/140‑2 (MQ=255) ttAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                  <  2:307849/140‑2 (MQ=255) ttAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                  <  1:300912/140‑2 (MQ=255)         tataCCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                        <  2:121003/126‑1 (MQ=255)         tataCCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                        >  1:121003/1‑126 (MQ=255)               aaaTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgc                                                                                                     <  2:335103/140‑1 (MQ=255)                aaTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgct                                                                                                    >  1:658184/1‑140 (MQ=255)                 aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTCCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgctg                                                                                                   >  1:879870/1‑140 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgctgct                                                                                                 >  1:626303/1‑140 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctg                                                                                                      >  2:727242/1‑135 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctg                                                                                                      <  1:727242/135‑1 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                                                       >  2:377668/1‑134 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                                                       <  1:377668/134‑1 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTCCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                                                       >  2:612937/1‑134 (MQ=255)                   gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTCCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                                                       <  1:612937/134‑1 (MQ=255)                     cACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTcc                                                                                               >  1:989785/1‑140 (MQ=255)                         aTTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGtt                                                                                           <  2:658184/140‑1 (MQ=255)                         aTTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGtt                                                                                           <  1:58070/140‑1 (MQ=255)                             tAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgc                                                                                       >  1:1048760/1‑140 (MQ=255)                              aGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgcg                                                                                      <  1:691803/140‑1 (MQ=255)                                    tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgc                                                                                >  1:1026655/1‑140 (MQ=255)                                    tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATG‑AACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgct                                                                               >  2:693952/1‑140 (MQ=255)                                     cTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgc                                                                                       >  1:444624/1‑132 (MQ=255)                                     cTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgc                                                                                       <  2:444624/132‑1 (MQ=255)                                     cTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgct                                                                               >  2:156699/1‑140 (MQ=255)                                     cTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgct                                                                               >  2:38779/1‑140 (MQ=255)                                        aaCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                                            <  1:73979/140‑1 (MQ=255)                                         aCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggct                                                                           >  2:1115474/1‑140 (MQ=255)                                             gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTtgtg                                                                                        >  1:471141/1‑123 (MQ=255)                                             gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTtgtg                                                                                        <  2:471141/123‑1 (MQ=255)                                                   atTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc                                                                 <  2:232161/140‑1 (MQ=255)                                                    ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCa                                                                <  1:325184/140‑1 (MQ=255)                                                       aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTc                                                             >  2:482272/1‑140 (MQ=255)                                                       aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTc                                                             >  1:739357/1‑140 (MQ=255)                                                       aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTc                                                             >  2:1140597/1‑140 (MQ=255)                                                       aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTc                                                             >  2:268548/1‑140 (MQ=255)                                                          cAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                                          <  2:468002/140‑1 (MQ=255)                                                            aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCtggctggc                                                                        <  2:503659/124‑1 (MQ=255)                                                            aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCtggctggc                                                                        >  1:503659/1‑124 (MQ=255)                                                             aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGAc                                                       >  1:108553/1‑140 (MQ=255)                                                                   ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGc                                                 <  2:1048760/140‑1 (MQ=255)                                                                                tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAg                                    >  1:835490/1‑140 (MQ=255)                                                                                  tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                              >  1:167968/1‑128 (MQ=255)                                                                                  tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                              <  2:167968/128‑1 (MQ=255)                                                                                  tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt                                  >  1:1174301/1‑140 (MQ=255)                                                                                      aCCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                              <  1:693952/140‑1 (MQ=255)                                                                                       ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgc                             >  2:596983/1‑140 (MQ=255)                                                                                       ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgc                             >  2:997303/1‑140 (MQ=255)                                                                                       ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgc                             >  1:1109115/1‑140 (MQ=255)                                                                                           gCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg                         <  2:739357/140‑1 (MQ=255)                                                                                            cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                        <  2:1109115/140‑1 (MQ=255)                                                                                             ttCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCa                       <  1:156699/140‑1 (MQ=255)                                                                                               ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACg                     >  2:1179880/1‑140 (MQ=255)                                                                                                 agagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGTTGATGCGCGGCACGCt                   >  1:97393/1‑140 (MQ=255)                                                                                                  gagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCtt                  >  1:804937/1‑140 (MQ=255)                                                                                                    gCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTa                >  2:925240/1‑140 (MQ=255)                                                                                                     cGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAt               >  1:1189068/1‑140 (MQ=255)                                                                                                       gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt             <  1:1115474/140‑1 (MQ=255)                                                                                                            acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGTGCGGCACGCTTTATTTTCTCg        <  1:1155865/140‑1 (MQ=255)                                                                                                            acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCg        <  1:997303/140‑1 (MQ=255)                                                                                                            acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCg        <  1:1140597/140‑1 (MQ=255)                                                                                                            acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCg        <  2:1019817/140‑1 (MQ=255)                                                                                                            acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCACGGCACGCTTTATTTTCTCg        <  1:482272/140‑1 (MQ=255)                                                                                                               gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTa     >  2:494371/1‑140 (MQ=255)                                                                                                               gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTa     >  2:544253/1‑140 (MQ=255)                                                                                                                  tgtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATcc  >  2:588837/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                        TTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCC  >  NC_000913/1053378‑1053629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑