Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,231,060	(A)6→7	coding (294/420 nt)	umuD →	DNA polymerase V protein UmuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,231,054	1	.	A	100.0%	343.6 / NA	96	V96V (GTG→GTA)	umuD	DNA polymerase V protein UmuD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (42/54);  total (42/54)

ACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTG‑AAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCT  >  NC_000913/1230917‑1231184                                                                                                                                           |                                                                                                                                   acttacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTG‑aa                                                                                                                                  >  2:175297/1‑140 (MQ=255)     acttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTG‑aaaaaa                                                                                                                              >  1:1065812/1‑140 (MQ=255)        tCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTG‑aaaaaaatt                                                                                                                           <  1:896224/140‑4 (MQ=255)               aGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTa                                                                                                                     >  2:728881/1‑140 (MQ=255)               aGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTa                                                                                                                     <  1:619027/140‑1 (MQ=255)               aGCAAGTGGGGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATGGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTa                                                                                                                     >  2:1137157/1‑140 (MQ=255)                     tggtgATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCg                                                                                                               >  1:1023628/1‑140 (MQ=255)                      ggtgATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGa                                                                                                              <  2:1065812/140‑1 (MQ=255)                      ggtgATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGa                                                                                                              <  2:125274/140‑1 (MQ=255)                          aTTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGt                                                                                                          >  2:1185612/1‑140 (MQ=255)                          aTTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGt                                                                                                          >  1:653749/1‑140 (MQ=255)                           ttCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTa                                                                                                         >  1:1079358/1‑140 (MQ=255)                           ttCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTa                                                                                                         <  1:514774/140‑1 (MQ=255)                              tATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATGTTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAg                                                                                                      >  2:1064556/1‑140 (MQ=255)                              tATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAg                                                                                                      >  2:601314/1‑140 (MQ=255)                               aTGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGc                                                                                                     >  2:489897/1‑140 (MQ=255)                                  attgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  2:240706/140‑1 (MQ=255)                                  attgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  2:508901/140‑1 (MQ=255)                                  attgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  1:1224389/140‑1 (MQ=255)                                  attgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  2:35240/140‑1 (MQ=255)                                  attgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCACCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  1:601314/140‑1 (MQ=255)                                      atGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTa                                                                                                         >  2:1087329/1‑129 (MQ=255)                                      atGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTa                                                                                                         <  1:1087329/129‑1 (MQ=255)                                          tggAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  >  2:343051/1‑132 (MQ=255)                                          tggAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTa                                                                                                  <  1:343051/132‑1 (MQ=255)                                            gAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGaa                                                                                        <  1:473799/140‑1 (MQ=255)                                            gAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGaa                                                                                        <  2:673/140‑1 (MQ=255)                                            gAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGaa                                                                                        <  2:1079358/140‑1 (MQ=255)                                            gAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGaa                                                                                        <  1:160053/140‑1 (MQ=255)                                                   tGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTCCGCCCGACGGTACAGCTTATTCCCATGAACAGcgcg                                                                                 <  2:439039/140‑1 (MQ=255)                                                      cGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                                                                                                <  2:851667/122‑1 (MQ=255)                                                      cGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                                                                                                >  1:851667/1‑122 (MQ=255)                                                       ggTGATTTACTGATTGTCGATGGCGCTATTCCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACt                                                                             <  2:596683/140‑1 (MQ=255)                                                       ggTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACt                                                                             >  1:1138228/1‑140 (MQ=255)                                                       ggTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACt                                                                             <  2:1143640/140‑1 (MQ=255)                                                        gTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTc                                                                            >  2:457168/1‑140 (MQ=255)                                                          gATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGc                                                                          <  1:457168/140‑1 (MQ=255)                                                           aTTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGcc                                                                         <  2:715148/140‑1 (MQ=255)                                                               aCTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCAtt                                                                     <  2:942765/140‑1 (MQ=255)                                                               aCTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCAtt                                                                     <  1:144988/140‑1 (MQ=255)                                                               aCTGATTGTCGAAAGCGCTATTACCGCCGGCCAGGGTGATATTGTAATCGCTGTTGTTGACGGAGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCAtt                                                                     <  2:1031100/140‑1 (MQ=255)                                                                 tGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCg                                                                           <  1:276284/132‑1 (MQ=255)                                                                 tGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCg                                                                           >  2:276284/1‑132 (MQ=255)                                                                 tGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAc                                                                   >  1:744371/1‑140 (MQ=255)                                                                  gATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAcc                                                                  <  1:756478/140‑1 (MQ=255)                                                                  gATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAcc                                                                  <  2:1138228/140‑1 (MQ=255)                                                                  gATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAcc                                                                  <  1:634961/140‑1 (MQ=255)                                                                  gATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAcc                                                                  <  1:175297/140‑1 (MQ=255)                                                                  gATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTAcc                                                                  <  2:653749/140‑1 (MQ=255)                                                                   aTTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCa                                                                 >  1:175151/1‑140 (MQ=255)                                                                   aTTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCa                                                                 >  2:488283/1‑140 (MQ=255)                                                                   aTTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTACCATGAACAGCGCGTACTCGCCCATTACCa                                                                 >  2:750244/1‑140 (MQ=255)                                                                    ttGTCGATAGCGCTATTACCGCCAGCCATGGTGGTATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACt                                                                             <  2:1160282/127‑1 (MQ=255)                                                                    ttGTCGATAGCGCTATTACCGCCAGCCATGGTGGTATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACt                                                                             >  1:1160282/1‑127 (MQ=255)                                                                     tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGATGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATc                                                               >  2:1004287/1‑140 (MQ=255)                                                                      gTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCAt                                                                <  2:677398/138‑1 (MQ=255)                                                                      gTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCAt                                                                >  1:677398/1‑138 (MQ=255)                                                                       tCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCag                                                             >  1:641258/1‑140 (MQ=255)                                                                          aTAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCagtag                                                          >  1:460953/1‑140 (MQ=255)                                                                          aTAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCagtag                                                          >  2:265807/1‑140 (MQ=255)                                                                                cTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGa                                                    <  1:778370/140‑1 (MQ=255)                                                                                cTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGa                                                    <  1:750244/140‑1 (MQ=255)                                                                                        gccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTgg                                            >  2:561212/1‑140 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGATGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGa                                           <  1:1004287/140‑1 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGa                                           <  1:377740/140‑1 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGa                                           >  2:337478/1‑140 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGa                                           <  1:489897/140‑1 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGa                                           <  2:175151/140‑1 (MQ=255)                                                                                         ccagccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGCAGTGAAGATACGCTGGa                                           <  2:1023628/140‑1 (MQ=255)                                                                                           agccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTATGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATg                                         >  2:1013348/1‑140 (MQ=255)                                                                                           agccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATg                                         >  1:507808/1‑140 (MQ=255)                                                                                            gccaTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGt                                        <  2:1126486/140‑1 (MQ=255)                                                                                               aTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCtt                                     <  1:1064556/140‑1 (MQ=255)                                                                                               aTGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCtt                                     <  2:460953/140‑1 (MQ=255)                                                                                                 ggTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTATGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtg                                   <  1:1013348/140‑1 (MQ=255)                                                                                                 ggTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtg                                   <  1:561212/140‑1 (MQ=255)                                                                                                     atatTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCtt                                     >  1:190612/1‑134 (MQ=255)                                                                                                     atatTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCtt                                     <  2:190612/134‑1 (MQ=255)                                                                                                       atTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtggtgtgg                             >  2:1129624/1‑140 (MQ=255)                                                                                                       atTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtggtgtgg                             >  1:785720/1‑140 (MQ=255)                                                                                                          gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGa                          >  1:591403/1‑140 (MQ=255)                                                                                                           tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGAt                         >  2:407854/1‑140 (MQ=255)                                                                                                            cATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATc                        <  1:1137157/140‑1 (MQ=255)                                                                                                             aTCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATcc                       >  2:1178178/1‑140 (MQ=255)                                                                                                             aTCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATcc                       >  2:583432/1‑140 (MQ=255)                                                                                                               cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCa                      >  2:857176/1‑139 (MQ=255)                                                                                                               cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCa                      <  1:857176/139‑1 (MQ=255)                                                                                                               cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCAc                     <  2:744371/140‑1 (MQ=255)                                                                                                                gctgctGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtg                                   >  1:779816/1‑125 (MQ=255)                                                                                                                gctgctGTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtg                                   <  2:779816/125‑1 (MQ=255)                                                                                                                     tgTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGAt                         <  2:103006/130‑1 (MQ=255)                                                                                                                     tgTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGAt                         >  1:103006/1‑130 (MQ=255)                                                                                                                     tgTTGACGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGtt               >  2:1086330/1‑140 (MQ=255)                                                                                                                           cGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCg         <  1:728881/140‑1 (MQ=255)                                                                                                                           cGGCGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCg         <  2:507808/140‑1 (MQ=255)                                                                                                                              cGAGTTTACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATg      >  2:513096/1‑140 (MQ=255)                                                                                                                                  tttACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCt  <  1:407854/140‑1 (MQ=255)                                                                                                                                  tttACGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCt  <  1:198367/140‑1 (MQ=255)                                                                                                                                      cGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCt  <  1:144186/136‑1 (MQ=255)                                                                                                                                      cGGTGAAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCt  >  2:144186/1‑136 (MQ=255)                                                                                                                                           |                                                                                                                                   ACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTG‑AAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCT  >  NC_000913/1230917‑1231184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑