Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,626	A→G	G385G (GGT→GGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,626	0	A	G	100.0%	177.7 / NA	51	G385G (GGT→GGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

GGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTT  >  NC_000913/64491‑64748                                                                                                                                         |                                                                                                                           ggTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAg                                                                                                                        <  1:620561/140‑1 (MQ=255) ggTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAg                                                                                                                        <  2:329350/140‑1 (MQ=255)   tGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAgcg                                                                                                                      >  2:1012882/1‑140 (MQ=255)   tGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAgcg                                                                                                                      >  2:792720/1‑140 (MQ=255)    gCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAgcgc                                                                                                                     >  1:685685/1‑140 (MQ=255)    gCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAgcgc                                                                                                                     >  1:695280/1‑140 (MQ=255)     cGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGcgcg                                                                                                                    >  2:1123013/1‑140 (MQ=255)               aCGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTcgc                                                                                                          >  1:434335/1‑140 (MQ=255)                cGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTcgcg                                                                                                         <  1:915740/140‑1 (MQ=255)                cGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTcgcg                                                                                                         <  1:251203/140‑1 (MQ=255)                cGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTcgcg                                                                                                         <  2:695280/140‑1 (MQ=255)                    tACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGaaa                                                                                                     >  2:929314/1‑140 (MQ=255)                     aCAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGaaaa                                                                                                    <  1:965480/140‑1 (MQ=255)                      cAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAAt                                                                                                   >  2:473323/1‑140 (MQ=255)                              tataGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGa                                                                                           >  2:533400/1‑140 (MQ=255)                                   tCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCaaa                                                                                      >  2:717064/1‑140 (MQ=255)                                   tCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCaaa                                                                                      >  1:61161/1‑140 (MQ=255)                                        caccagcacTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATgccg                                                                                 >  2:515703/1‑140 (MQ=255)                                              cacTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCg                                                                           <  1:515703/140‑1 (MQ=255)                                              cacTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCg                                                                           <  1:569025/140‑1 (MQ=255)                                                   aaTCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAAccg                                                                      <  2:61161/140‑1 (MQ=255)                                                    aTCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAAccgc                                                                     <  1:473323/140‑1 (MQ=255)                                                        tAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCgtg                                                                 >  2:196874/1‑140 (MQ=255)                                                         aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCgtgt                                                                >  2:155590/1‑140 (MQ=255)                                                          aaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTc                                                               <  1:717064/140‑1 (MQ=255)                                                          aaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTc                                                               <  1:339651/140‑1 (MQ=255)                                                              ccTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTc                                                           <  1:1123013/140‑1 (MQ=255)                                                                tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCa                                                         >  1:652749/1‑140 (MQ=255)                                                                tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCa                                                         >  1:377055/1‑140 (MQ=255)                                                                tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCa                                                         >  2:74471/1‑140 (MQ=255)                                                                tGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCa                                                         >  1:124236/1‑140 (MQ=255)                                                                 ggCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCAc                                                        >  2:344697/1‑140 (MQ=255)                                                                  gCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCAcc                                                       >  2:1026627/1‑140 (MQ=255)                                                                     gTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGc                                                    <  1:155590/140‑1 (MQ=255)                                                                        gccccATCAGGTCGCCGCGAGGGC‑GGCGGGGGGCCGGCCCTTCCCCGAGATAGGGCGCGCCATGGCCAGCGAGATGCTTTGGCGAAAAAAAGAGATGACAAAACGCCCCCCCCGAACCGCCGTGTCGGTCCACCGGCAgg                                                 <  2:308888/137‑1 (MQ=255)                                                                        aacccATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAgg                                                 <  2:955339/137‑1 (MQ=255)                                                                        aaTCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAgg                                                 <  1:180843/140‑1 (MQ=255)                                                                        aaTCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAgg                                                 <  2:652749/140‑1 (MQ=255)                                                                        aaTCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAgg                                                 <  1:929314/140‑1 (MQ=255)                                                                              tCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTc                                           <  1:1026627/140‑1 (MQ=255)                                                                                   tAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAATCGCCGTGTCGGTCCACCGGCAGGCCGtt                                            >  1:789442/1‑134 (MQ=255)                                                                                   tAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAATCGCCGTGTCGGTCCACCGGCAGGCCGtt                                            <  2:789442/134‑1 (MQ=255)                                                                                    aGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGtc                                     >  1:308888/1‑139 (MQ=255)                                                                                           ggggCTGGCGTGCGG‑GGGCTCTTCGCCGAGATTAGGCGCGACATAGCCAGCGGGATGCATTCGGGGAAAATAGAGATGACCAAAGGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                              <  2:434335/139‑1 (MQ=255)                                                                                           aGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                              <  1:668257/140‑1 (MQ=255)                                                                                             ggCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCg                            >  2:1196143/1‑140 (MQ=255)                                                                                                  gCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGt                               <  1:1841/132‑1 (MQ=255)                                                                                                  gCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGt                               >  2:1841/1‑132 (MQ=255)                                                                                                    gTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTaaaa                     <  1:1196143/140‑1 (MQ=255)                                                                                                       cgg‑cggCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATg                  >  2:630201/1‑140 (MQ=255)                                                                                                             gcACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATg             >  1:464071/1‑140 (MQ=255)                                                                                                                        agaTTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGtttt  <  1:74471/140‑1 (MQ=255)                                                                                                                        agaTTAGGCGCGACATAGCCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGtttt  <  2:734819/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           GGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGG‑CGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTT  >  NC_000913/64491‑64748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑