Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,704,371	A→G	T313T (ACT→ACC)	lepB ←	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,704,371	0	A	G	100.0%	165.9 / NA	46	T313T (ACT→ACC)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/19);  total (27/19)

TACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTAT  >  NC_000913/2704238‑2704506                                                                                                                                      |                                                                                                                                        taCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGc                                                                                                                                   >  1:535190/1‑140 (MQ=255)      aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTc                                                                                                                              >  2:350934/1‑140 (MQ=255)      aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTc                                                                                                                              >  2:676687/1‑140 (MQ=255)       acgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCg                                                                                                                             <  1:679254/140‑1 (MQ=255)       acgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCg                                                                                                                             <  1:510389/140‑1 (MQ=255)        cgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGc                                                                                                                            >  1:98390/1‑140 (MQ=255)         gacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGcc                                                                                                                           <  2:697348/140‑1 (MQ=255)                              cGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCAt                                                                                                      <  2:535190/140‑1 (MQ=255)                               gggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATc                                                                                                     >  1:52951/1‑140 (MQ=255)                                       tATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGc                                                                                             <  1:528225/140‑1 (MQ=255)                                           tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGtt                                                                                         >  2:19496/1‑140 (MQ=255)                                                     ttCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccga                                                                               >  2:648622/1‑140 (MQ=255)                                                       cACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCgaccgacc                                                                             >  2:38192/1‑140 (MQ=255)                                                             gTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                       >  1:1156713/1‑140 (MQ=255)                                                                      aCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGc                                                              >  2:525523/1‑140 (MQ=255)                                                                       cGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGca                                                             <  2:98390/140‑1 (MQ=255)                                                                        ggCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcac                                                            >  2:667853/1‑140 (MQ=255)                                                                        ggCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcac                                                            >  2:1004116/1‑140 (MQ=255)                                                                        ggCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcac                                                            >  1:1095993/1‑140 (MQ=255)                                                                           gACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcaca                                                           >  2:660786/1‑138 (MQ=255)                                                                           gACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcaca                                                           <  1:660786/138‑1 (MQ=255)                                                                           gACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa                                                         <  1:770634/140‑1 (MQ=255)                                                                              aaCGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGcc                                                      <  2:1156713/140‑1 (MQ=255)                                                                                    aaCGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTa                                                >  2:846919/1‑140 (MQ=255)                                                                                        aaGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACgg                                            >  1:306481/1‑140 (MQ=255)                                                                                              gCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTcc                                      >  2:1135603/1‑140 (MQ=255)                                                                                               cTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCcg                                     <  2:1079839/140‑1 (MQ=255)                                                                                               cTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCcg                                     >  2:975695/1‑140 (MQ=255)                                                                                                  ttAATGGATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCgcgc                                  <  1:414167/140‑1 (MQ=255)                                                                                                        gATGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCtgttgt                            >  2:116075/1‑140 (MQ=255)                                                                                                         aTGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTc                           >  2:142547/1‑140 (MQ=255)                                                                                                          tGCCGCCAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTcg                          <  2:306481/140‑1 (MQ=255)                                                                                                            ccgccAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGTCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTcgcg                        >  1:739272/1‑140 (MQ=255)                                                                                                            ccgccAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTcgcg                        >  1:990259/1‑140 (MQ=255)                                                                                                            ccgccAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTcgcg                        >  2:999375/1‑140 (MQ=255)                                                                                                            ccgccAATGCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCACCAGTAACGGCTGTCCGCGCTGTTGTcgcg                        >  1:10598/1‑140 (MQ=255)                                                                                                                    gCGACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGc                <  1:846919/140‑1 (MQ=255)                                                                                                                      gACTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGccc              <  2:52951/140‑1 (MQ=255)                                                                                                                       aCTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCca             <  1:975695/140‑1 (MQ=255)                                                                                                                       aCTTAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCca             <  1:525523/140‑1 (MQ=255)                                                                                                                         ttAAGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCcatc           <  1:1135603/140‑1 (MQ=255)                                                                                                                           aaGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCcatcat         >  1:436626/1‑140 (MQ=255)                                                                                                                            aGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATg        <  2:436626/140‑1 (MQ=255)                                                                                                                            aGCGCAGACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATg        <  1:810184/140‑1 (MQ=255)                                                                                                                                  gACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTAt  >  1:383915/1‑140 (MQ=255)                                                                                                                                  gACCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTAt  >  1:228295/1‑140 (MQ=255)                                                                                                                                   aCCGGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCca             <  2:561984/128‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTAT  >  NC_000913/2704238‑2704506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑