Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,750,543	T→C	T56A (ACC→GCC)	grpE ←	nucleotide exchange factor GrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,750,543	0	T	C	100.0%	231.6 / NA	63	T56A (ACC→GCC)	grpE	nucleotide exchange factor GrpE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/30);  total (33/30)

TCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCT  >  NC_000913/2750410‑2750673                                                                                                                                      |                                                                                                                                   tcaatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGc                                                                                                                              >  2:532278/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTggg                                                                                                                               <  1:756944/138‑1 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTggg                                                                                                                               >  2:756944/1‑138 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:446990/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  1:993500/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:161346/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:176502/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  1:186083/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:496985/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:802844/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:875861/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:884086/1‑140 (MQ=255)  caatcaCCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:890271/1‑140 (MQ=255)  caatcaCCAGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCt                                                                                                                             >  2:1143044/1‑140 (MQ=255)           gcagcaATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTcagccagc                                                                                                                    >  1:524231/1‑140 (MQ=255)               caATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAG‑TCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGc                                                                                                               >  1:208770/1‑140 (MQ=255)                    cGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCg                                                                                                           <  1:890271/140‑1 (MQ=255)                        gatgaATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAG‑TCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGAtt                                                                                                      <  2:208770/140‑1 (MQ=255)                            aaTTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGc                                                                                                   <  2:993500/140‑1 (MQ=255)                                      gcgcGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCa                                                                                         >  1:21780/1‑140 (MQ=255)                                          gAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgc                                                                                     <  2:1137385/140‑1 (MQ=255)                                          gAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgc                                                                                     <  1:496985/140‑1 (MQ=255)                                          gAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgc                                                                                     <  2:186083/140‑1 (MQ=255)                                          gAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgc                                                                                     <  1:802844/140‑1 (MQ=255)                                            aTTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgcgc                                                                                   >  1:684382/1‑140 (MQ=255)                                             tttGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgcgcg                                                                                  <  1:108914/140‑1 (MQ=255)                                             tttGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATcgcgcg                                                                                  <  1:1143044/140‑1 (MQ=255)                                               tgtgGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGa                                                                                <  1:532278/140‑1 (MQ=255)                                                   ggCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCTTGGGCTTCAGCCAGCTGAGCTTCGAGATTCACAACTTTTTCATCGCGCGGATCCa                                                                            >  2:305785/1‑140 (MQ=255)                                                        tttCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGc                                                                       >  1:989975/1‑140 (MQ=255)                                                             atatCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTcagc                                                                  <  2:822625/140‑1 (MQ=255)                                                                tCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGa                                                               >  2:554424/1‑140 (MQ=255)                                                                tCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGa                                                               <  1:1091020/140‑1 (MQ=255)                                                                     ttCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCtt                                                          >  1:957568/1‑140 (MQ=255)                                                                      tCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTc                                                         >  2:1035424/1‑140 (MQ=255)                                                                      tCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTc                                                         <  2:957568/140‑1 (MQ=255)                                                                        aGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTg                                                       >  1:172238/1‑140 (MQ=255)                                                                          tACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGc                                                     >  1:1031651/1‑140 (MQ=255)                                                                          tACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGc                                                     >  2:1194187/1‑140 (MQ=255)                                                                              acgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCTCGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCaa                                                 <  2:989975/140‑1 (MQ=255)                                                                              acgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCATCAGAAGCTTCTGGCTCaa                                                 <  2:684382/140‑1 (MQ=255)                                                                              acgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCaa                                                 <  2:172238/140‑1 (MQ=255)                                                                              acgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCaa                                                 <  1:875861/140‑1 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGa                                                               >  1:79482/1‑125 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGa                                                               <  2:79482/125‑1 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCtt                                                          <  1:524494/130‑1 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCtt                                                          >  2:524494/1‑130 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAAc                                                >  1:752564/1‑140 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAAc                                                >  1:776322/1‑140 (MQ=255)                                                                               cgacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAAc                                                >  1:178969/1‑140 (MQ=255)                                                                                gacgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACt                                               <  1:619933/140‑1 (MQ=255)                                                                                  cgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGTAGCTTCTGGCTCAACTGc                                             >  1:195383/1‑140 (MQ=255)                                                                                  cgCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGc                                             >  2:593139/1‑140 (MQ=255)                                                                                    cAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCt                                           <  2:776322/140‑1 (MQ=255)                                                                                       gTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCaa                                        <  1:446990/140‑1 (MQ=255)                                                                                            ccATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATctct                                   <  1:554424/140‑1 (MQ=255)                                                                                            ccATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATctct                                   <  2:178969/140‑1 (MQ=255)                                                                                             cATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCtt                                  <  1:161346/140‑1 (MQ=255)                                                                                                        ttACACGCAAAATGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATcc                       <  1:176502/140‑1 (MQ=255)                                                                                                                  aaTGCCGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAAttt             >  1:928799/1‑140 (MQ=255)                                                                                                                      ccGTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATttcttc         <  1:593139/140‑1 (MQ=255)                                                                                                                        gTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCg       <  1:824654/140‑1 (MQ=255)                                                                                                                        gTCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCg       <  1:707810/140‑1 (MQ=255)                                                                                                                         tCACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCgg      >  2:1008054/1‑140 (MQ=255)                                                                                                                          cACGTTCACGGGCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg     >  2:1098212/1‑140 (MQ=255)                                                                                                                                   gggCCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCt  <  2:394343/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   TCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTGTGGGCTTTTTCAATATCCAGTTCAGTACGACGACGCAGGTTTTCCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCT  >  NC_000913/2750410‑2750673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑