Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,899	T→C	intergenic (+227/‑330)	ppnN → / → sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,899	0	T	C	100.0%	334.7 / NA	90	intergenic (+227/‑330)	ppnN/sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/47);  total (43/47)

ACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCT  >  NC_000913/2927765‑2928032                                                                                                                                       |                                                                                                                                      accaccCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGa                                                                                                                                  >  2:508880/1‑140 (MQ=255)    accCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAttt                                                                                                                               >  2:662083/1‑140 (MQ=255)     cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAtttt                                                                                                                              >  2:703053/1‑140 (MQ=255)     cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAtttt                                                                                                                              >  2:819529/1‑140 (MQ=255)     cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAtttt                                                                                                                              >  1:172990/1‑140 (MQ=255)      ccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAttttt                                                                                                                             >  2:465095/1‑140 (MQ=255)      ccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGAttttt                                                                                                                             >  1:1144990/1‑140 (MQ=255)           cgcgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGt                                                                                                                        <  1:972215/140‑1 (MQ=255)           cgcgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGt                                                                                                                        <  1:508880/140‑1 (MQ=255)            gcgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGtt                                                                                                                       <  2:674790/140‑1 (MQ=255)             cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  2:542372/140‑1 (MQ=255)             cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  1:1050706/140‑1 (MQ=255)             cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  2:948951/140‑1 (MQ=255)             cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  2:683496/140‑1 (MQ=255)             cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  1:5191/140‑1 (MQ=255)              gACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCa                                                                                                                     <  1:1025921/140‑1 (MQ=255)               aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTcga                                                                                                                    >  2:1133520/1‑138 (MQ=255)               aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCaa                                                                                                                    <  1:662083/140‑1 (MQ=255)               aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCaa                                                                                                                    <  2:172990/140‑1 (MQ=255)               aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCaa                                                                                                                    <  1:717796/140‑1 (MQ=255)               aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCaa                                                                                                                    <  1:819529/140‑1 (MQ=255)                cAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      <  2:40366/137‑1 (MQ=255)                cAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTc                                                                                                                      >  1:40366/1‑137 (MQ=255)                 aaaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  <  2:864275/140‑1 (MQ=255)                 aaaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  <  1:703053/140‑1 (MQ=255)                        atcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  <  1:399081/133‑1 (MQ=255)                        atcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  >  2:399081/1‑133 (MQ=255)                          cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGtt                                                                                                                       >  1:875392/1‑126 (MQ=255)                          cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGtt                                                                                                                       <  2:875392/126‑1 (MQ=255)                          cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACatatat                                                                                                         >  1:213793/1‑140 (MQ=255)                            caGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                       >  1:502700/1‑140 (MQ=255)                            caGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                       <  1:915418/140‑1 (MQ=255)                              gTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     <  1:1174975/140‑1 (MQ=255)                              gTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     <  1:1048509/140‑1 (MQ=255)                              gTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     <  2:502700/140‑1 (MQ=255)                                 aaTATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  <  2:33340/124‑1 (MQ=255)                                 aaTATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAAcc                                                                                                                  >  1:33340/1‑124 (MQ=255)                                  atatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     >  2:1190047/1‑136 (MQ=255)                                  atatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     <  1:1190047/136‑1 (MQ=255)                                   tatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGccc                                                                                                <  2:334113/140‑1 (MQ=255)                                   tatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGccc                                                                                                <  2:1144990/140‑1 (MQ=255)                                      gTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCgg                                                                                             <  1:61280/140‑1 (MQ=255)                                       tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                            >  1:154292/1‑140 (MQ=255)                                       tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                            >  1:845045/1‑140 (MQ=255)                                       tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                            >  1:1142831/1‑140 (MQ=255)                                         aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaag                                                                                          >  2:151511/1‑140 (MQ=255)                                         aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaag                                                                                          >  2:353404/1‑140 (MQ=255)                                         aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaag                                                                                          >  1:198575/1‑140 (MQ=255)                                               tttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     <  2:395131/123‑1 (MQ=255)                                               tttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                     >  1:395131/1‑123 (MQ=255)                                                ttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAg                                                                                   >  1:1118179/1‑140 (MQ=255)                                                ttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAg                                                                                   <  1:146024/140‑1 (MQ=255)                                                    gTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGt                                                                               >  1:1036375/1‑140 (MQ=255)                                                       gCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGt                                                                            >  2:476752/1‑140 (MQ=255)                                                       gCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCACTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGt                                                                            >  1:6326/1‑140 (MQ=255)                                                         aaCGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGttt                                                                          >  2:217740/1‑140 (MQ=255)                                                           cGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTaa                                                                        <  2:1142831/140‑1 (MQ=255)                                                           cGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTaa                                                                        <  2:845045/140‑1 (MQ=255)                                                               aaCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATggg                                                                    <  1:353404/140‑1 (MQ=255)                                                                        cTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg                                                           <  1:476752/140‑1 (MQ=255)                                                                            tttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGc                                                                   <  2:930817/128‑1 (MQ=255)                                                                            tttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGc                                                                   >  1:930817/1‑128 (MQ=255)                                                                                aTAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaaa                                                   <  2:1036375/140‑1 (MQ=255)                                                                                aTAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaaa                                                   <  1:234684/140‑1 (MQ=255)                                                                                     ccGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtctc                                              >  2:903125/1‑140 (MQ=255)                                                                                     ccGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtctc                                              >  1:702354/1‑140 (MQ=255)                                                                                        tATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCttt                                           >  2:1035329/1‑140 (MQ=255)                                                                                         aTTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCtttt                                          >  1:603816/1‑140 (MQ=255)                                                                                          ttttATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTa                                         >  2:99450/1‑140 (MQ=255)                                                                                          ttttATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTa                                         >  1:576009/1‑140 (MQ=255)                                                                                                cGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTg                                   <  2:1199297/140‑1 (MQ=255)                                                                                                cGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTg                                   >  2:864026/1‑140 (MQ=255)                                                                                                  cTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAt                                 <  1:1035329/140‑1 (MQ=255)                                                                                                    aTGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAttt                               >  1:115581/1‑140 (MQ=255)                                                                                                    aTGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAttt                               <  2:115581/140‑1 (MQ=255)                                                                                                     tGAAAAAGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                              <  1:1133520/140‑1 (MQ=255)                                                                                                           aGAAATTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGt                        <  1:188261/140‑1 (MQ=255)                                                                                                             aaaTTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaa                      <  2:1118179/140‑1 (MQ=255)                                                                                                             aaaTTATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaa                      >  1:273061/1‑140 (MQ=255)                                                                                                                 tATCGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCg                  <  2:603816/140‑1 (MQ=255)                                                                                                                    cGCCATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTg               >  1:440844/1‑140 (MQ=255)                                                                                                                      ccATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                              <  1:172058/123‑1 (MQ=255)                                                                                                                      ccATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                              >  2:172058/1‑123 (MQ=255)                                                                                                                       cATGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGa            >  2:1107018/1‑140 (MQ=255)                                                                                                                        aTGATTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGAt           <  2:440844/140‑1 (MQ=255)                                                                                                                           aTTAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAat        >  2:415105/1‑140 (MQ=255)                                                                                                                            ttAACTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc       <  2:206119/140‑1 (MQ=255)                                                                                                                              aaCTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatcta     >  2:343565/1‑140 (MQ=255)                                                                                                                                cTAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTCTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctatc   >  2:802431/1‑140 (MQ=255)                                                                                                                                 tAAAAGCATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctatct  <  2:198575/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCT  >  NC_000913/2927765‑2928032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑